IDBR: Development of an Adaptive Optic Scanning Laser Microscope for Deep Tissue Imaging

在天文学领域，自适应光学用于校正星光穿过地球大气层时发生的像差：折射率的动态变化使光线发生反射，导致恒星“闪烁”。“在大型地面望远镜中使用的自适应光学系统包括一个波前传感器，用于测量“引导星星”的像差，“引导星星”是感兴趣物体视场中的天然或人工点源参考信标，自适应光学用于天文学中获得更清晰的天体图像，外层空间物体将重新应用于生物成像领域，以校正细胞内折射率变化引起的图像畸变。荧光珠将被用作参考信标，并被植入感兴趣的对象附近，例如细胞核。预期的结果是校正高度像差的图像，从而获得由成像系统的衍射设定的分辨率极限。一旦开发出来，显微镜将在一个新的研究中心，由加州再生医学研究所（www.cirm.ca.gov）资助。

In the field of astronomy, adaptive optics are used to correct for the image aberrations that occur as starlight passes through the Earth's atmosphere: Dynamic changes in the refractive index refract the light, causing stars to "twinkle." The adaptive optics that are used in large terrestrial telescopes consist of a wavefront sensor to measure the aberrations of a "guide star," a natural or artificial point source reference beacon in the field of view of the object of interest, and a deformable mirror that can dynamically change its shape correct the image to restore diffraction limited imaging.The adaptive optics used in astronomy to obtain clearer images of celestial bodies in outer space will be re-applied in the field of biological imaging to correct image aberrations from changes in the refractive index within a cell. A fluorescent bead will be used as the reference beacon and implanted near the object of interest, such as the nucleus of a cell. Expected outcome is the correction of highly aberrated images, thereby obtaining the resolution limit set by diffraction of the imaging system. Once developed, the microscope will be available in a new research center, funded by the California Institute for Regenerative Medicine (www.cirm.ca.gov).