High Resolution Magnetophoresis using Multilayered Magnetic Nanodisks

ECCS-0901849：多层磁性纳米磁盘的高分辨率磁渗透技术摘要本研究的目的是证明利用多层磁性纳米磁盘作为标记物，可以实现高分辨率的磁性多路生物分离。这种新的磁致伸展方法允许根据其不同的磁性连续分离尺寸小于100 nm的磁性纳米盘。智能优点：所提出的多层纳米盘由两个由非磁性层和两个盖层隔开的磁性层组成，可以提供大范围的磁性可调，这导致了与常用的超顺磁性氧化铁纳米颗粒相比的两个重要优势：开启了磁多路复用的新可能性，并填补了从30 nm到200 nm的超顺磁性纳米颗粒尺寸间隙，为生物分离提供了高矩磁标签。在微流控装置中，这些纳米盘将受到以三角波形式随时间变化的磁场。具有不同饱和场的纳米盘可以连续分离。由于标签的磁性和磁场都是已知的，整个分离过程可以很容易地建模，这将为我们提供一个方便的工具来优化不同类型的分离条件。更广泛的影响：分离是任何生化分析的重要组成部分。提出的分离方法模仿了广泛使用的电泳法，但用磁场取代了电场。磁场不干扰生物过程和材料，可以在外部施加，而不需要磁体与任何液体进行物理接触。磁力与离子强度、pH或表面电荷无关。由此可见，这一提议的方法如果成功，将为生物分离提供一种重要的替代方案。此外，它还可以将有价值的多路复用引入到许多高灵敏的生物传感检测方法中。这两者都将在许多生物和生物医学研究领域产生广泛的影响。此外，该项目还提供了一个出色的场所，将研究活动与对大学生和研究生的教育和培训结合起来，纳米生物技术被认为是美国在全球经济竞争和国家安全方面最有潜力的技术之一。将创建三个实验模块，用于本科纳米生物技术教学的新实践方法；？以实验为导向的即时教学？此外，通过德克萨斯大学阿灵顿分校现有的三个夏令营，数百名K-12年级的学生将接触到这项研究。其中，约30%是西班牙裔学生。学生肯定会受到积极的影响，并可能激发他们对理工科的兴趣。

ECCS-0901849: High Resolution Magnetophoresis Using Multilayered Magnetic NanodisksAbstractThe objective of this research is to demonstrate that a high resolution magnetic multiplexed bioseparation can be achieved by using multilayered magnetic nanodisks as labels. This new magnetophoretic approach allows a continuous separation of sub-100 nm sized magnetic nanodisks according to their distinct magnetic properties.Intellectual Merit: The proposed multilayered nanodisks, consisting of two magnetic layers separated by a non-magnetic layer with two capping layers, can provide a wide-range tunability of magnetic properties, which results in two important advantages over commonly used superparamagnetic iron oxide nanoparticles: opening up the new possibility of magnetic multiplexing and filling the superparamagnetic nanoparticle size gap, from 30 nm to 200 nm, to provide high-moment magnetic labels for bioseparation. In a microfluidic arrangement, these nanodisks will be subjected to a magnetic field varying with time in a triangle wave format. Nanodisks with different saturation fields can be continuously separated. Since magnetic properties of labels and magnetic fields are exactly known, the whole separation process can be easily modeled, which will provide us a convenient tool to optimize conditions for different kind of separations. Broader Impacts: Separation is an important part of any biochemical analysis. The proposed separation method mimics widely-used electrophoresis, but replacing electric field with magnetic field. Magnetic field does not interfere with biological process and materials, and can be applied externally without physical contact between magnet and any liquid. Magnetic forces are independent on ionic strength, pH or surface charges. It can be seen that this proposed approach, if successful, will provide an important alternative for bioseparation. Moreover, it can introduce valuable multiplexing into many highly sensitive detection methods for biosensing. Both of these will have a broad impact in many biological and biomedical research fields. Also, this project offers an outstanding venue to integrate research activities with the education and training for college and graduate students in nanobiotechnology, which is considered as one of the most potentially valuable technologies for U.S. in the global economic competition and for national security. Three experiment modules will be created for a new practicum approach on undergraduate nanobiotechnology teaching; ?experiment-oriented just-in-time teaching?. In addition, through three existing summer camps at UT Arlington, several hundreds of K-12 students will be exposed to this research. Among them, about thirty percent are Hispanic students. The students will certainly be affected positively and may spark their interest in science and engineering.