High-speed three dimensional coherent nonlinear imaging with temporal-focus depth scanned holography

具有时间聚焦深度扫描全息技术的高速三维相干非线性成像

• 批准号: 0901870
• 负责人: Randy Bartels
• 金额: $ 35万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0901870&HistoricalAwards=false
• 关键词: speed; three; dimensional; coherent; nonlinear

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."Coherent nonlinear laser microscopy (CNLM) such as third harmonic (THG) and second harmonic (SHG) generation microscopes are widely used imaging tools. However, their application to date has eluded systems requiring rapid image acquisition. We propose to develop a method for rapidly acquiring three-dimensional (3D) images formed by nonlinear microscopy by means of digital holography coupled with rapid spatio-temporal scanning of the driving ultrafast laser pulses. Intellectual Merits: The proposed research will develop these capabilities which can be used to study fast dynamics of many processes. Making use of recent advances in spatio-temporal focusing, it will be possible to develop new forms of very high speed 3D imaging with coherent nonlinear contrast mechanisms. We will build an understanding of the capabilities of 3D nonlinear imaging and will better understand how to optimize both transverse and longitudinal (axial) resolution, while obtaining 3D information about an object.Broader Impacts: This research will primarily focus on THG microscopy; but the technique is applicable to other coherent nonlinear microscopes, such as second-harmonic generation (SHG) and coherent anti-Stokes Raman spectroscopy (CARS). Increased acquisition rates made possible with holographic microscopy will permit the study of micro- to millisecond-timescale dynamics. Undergraduate researchers will be involved in all aspects of this project focusing on research areas of ultrafast and nonlinear optics, and microscopy. Research findings will be incorporated into courses taught by the PI, particularly Ultrafast Optics and Nonlinear Optics. In addition, the PI will continue revising and delivering his "optics show" for K-2nd graders.

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。相干非线性激光显微镜（CNLM），如三次谐波（THG）和二次谐波（SHG）生成显微镜是广泛使用的成像工具。然而，迄今为止，它们的应用已经避开了需要快速图像采集的系统。我们建议开发一种方法，用于快速获取三维（3D）图像形成的非线性显微镜通过数字全息术与驱动超快激光脉冲的快速时空扫描耦合。智力优势：拟议的研究将开发这些能力，可用于研究许多过程的快速动态。利用时空聚焦的最新进展，将有可能开发具有相干非线性对比度机制的新形式的非常高速的3D成像。我们将了解三维非线性成像的能力，并将更好地了解如何优化横向和纵向（轴向）分辨率，同时获得有关物体的三维信息。但是该技术可应用于其它相干非线性显微镜，例如二次谐波产生（SHG）和相干反斯托克斯拉曼光谱（汽车）。全息显微镜使获取率的提高成为可能，这将允许研究微秒到毫秒的时间尺度动力学。本科研究人员将参与该项目的各个方面，重点是超快和非线性光学和显微镜的研究领域。研究成果将被纳入PI教授的课程，特别是超快光学和非线性光学。此外，PI将继续修改和提供他的“光学显示”的K-2年级学生。