Role of beta1-integrin signaling in lymphatic vessel formation

β1-整合素信号在淋巴管形成中的作用

• 批准号: 192924567
• 负责人: Professor Dr. Eckhard Lammert
• 金额: --
• 依托单位: Institut für Stoffwechselphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/192924567?language=en
• 关键词: Role; beta1; integrin; signaling; lymphatic

Fluid leaks out of most vascular beds and increases the interstitial fluid pressure within a given tissue. Therefore, lymphatic vessels absorb this fluid and bring it back to the vascular system. This process is mediated by an interaction of lymphatic endothelial cells with the extracellular matrix (ECM) that opens the lymphatic vessels, whenever the interstitial fluid pressure rises. However, even though lymphatic endothelial cells express many beta1-integrin receptors for ECM proteins, little is known about the in vivo role of the beta1-integrin in lymphatic vessels. Thus we want to investigate the beta1-integrin and its signaling pathway in lymphatic vessels by using mice that lack the beta1-integrin and integrin-linked kinase (ILK) in their endothelial cells. Our current work warrants this study by showing that beta1-integrin deficient mice have severe defects in lymphatic vessel formation. We also plan to analyze how beta1-integrin and ILK regulate VEGF receptor-3 signaling in lymphatic endothelial cells in vitro and in vivo.

液体从大多数血管床中渗漏出来，并增加给定组织内的间质液压力。因此，淋巴管吸收这些液体并将其带回血管系统。这一过程是由淋巴管内皮细胞与细胞外基质（ECM）的相互作用介导的，每当间质液压力升高时，ECM就会打开淋巴管。然而，尽管淋巴管内皮细胞表达许多ECM蛋白的β 1-整联蛋白受体，但对β 1-整联蛋白在淋巴管中的体内作用知之甚少。因此，我们希望通过使用内皮细胞中缺乏β 1-整合素和整合素连接激酶（ILK）的小鼠来研究β 1-整合素及其在淋巴管中的信号通路。我们目前的工作证明，β 1-整合素缺陷的小鼠在淋巴管形成方面存在严重缺陷。我们还计划分析β 1-整合素和ILK如何在体外和体内调节淋巴管内皮细胞中VEGF受体-3信号传导。

项目成果

Mechanoinduction of lymph vessel expansion

• DOI: 10.1038/emboj.2011.456
• 发表时间: 2012-02-15
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Planas-Paz, Lara;Strilic, Boris;Lammert, Eckhard
• 通讯作者: Lammert, Eckhard

Mechanosensing in developing lymphatic vessels.

• DOI: 10.1007/978-3-7091-1646-3_3
• 发表时间: 2014
• 期刊: Advances in anatomy, embryology, and cell biology
• 作者: L. Planas-Paz;E. Lammert