Affinity Protein Capture by Immobilized DNA at Surfaces

通过表面固定化 DNA 捕获亲和蛋白

• 批准号: 0911108
• 负责人: Linda McGown
• 金额: $ 37.92万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0911108&HistoricalAwards=false
• 关键词: Affinity; Protein; Capture; Immobilized; DNA

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)." The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry supports the research program of Prof. Linda McGown of Rensselaer Polytechnique Institute. Prof. McGown and her students will study the processes that govern affinity protein capture at aptamer-modified surfaces using affinity MALDI mass spectrometry in combination with surface plasmon resonance (SPR) spectroscopy and imaging, and will apply the knowledge gained to enable the design of improved aptamers. The proposed study has the potential to greatly impact the field of aptamer design and facilitate the use of aptamers as recognition components in a wide range of chemical and biological applications. The project will provide excellent training opportunities to graduate and undergraduate students in a highly interdisciplinary area at the chemical biology interface. Prof. McGown and her students will also participate in the Science Technology Entry Program (STEP) at RPI, which aims to attract and retain high school students from underrepresented and economically disadvantaged groups in science and engineering fields.

该奖项是根据2009年《美国复苏和再投资法案》(公法111-5)资助的。化学系的分析和表面化学(ASC)计划支持伦斯勒理工学院的琳达·麦克高恩教授的研究计划。McGown教授和她的学生将使用亲和MALDI质谱仪结合表面等离子体共振(SPR)光谱和成像来研究在适体修饰的表面捕捉亲和蛋白的过程，并将应用所获得的知识来设计改进的适体。拟议的研究有可能极大地影响适体设计领域，并促进适体作为识别成分在广泛的化学和生物应用中的使用。该项目将为化学生物学界面的高度跨学科领域的研究生和本科生提供极好的培训机会。McGown教授和她的学生还将参加RPI的科学技术进入计划(STEP)，该计划旨在吸引和留住科学和工程领域代表不足和经济困难群体的高中生。