Lifetime-Resolved Fluorescence-Detected Circular Dichroism

寿命分辨荧光检测圆二色性

• 批准号: 9111928
• 负责人: Linda McGown
• 金额: $ 20.14万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-15 至 1995-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9111928&HistoricalAwards=false
• 关键词: Lifetime; Resolved; Fluorescence; Detected; Circular

This research, supported by the Analytical and Surface Chemistry Program in the Chemistry Division, will study the rate of association between chiral molecules and both chiral and achiral hosts using "lifetime-resolved fluorescence- detected circular dichroism" (LRFDCD), a method which can monitor the change in light polarization during a reaction. Light polarization and fluorescence lifetime can both be related to the speed and extent of reaction. Among the systems to be characterized are antibody-antigen interactions in immunochemical methods of analysis, solubilization of molecules in organized media, and interactions between chiral solutes and chromatographic phases in enantiomeric separations. %%% This research is concerned with the interactions of guest molecules with the molecular components of host cavities. Of particular interest are the effects of the shapes of the guests and of the host cavities on these interactions. Chiral and achiral hosts and chiral and achiral guests will be studied using a new technique called "time-resolved fluorescence-detected circular dichroism." The results will be applicable to chromatomatographic separations of chiral molecules and to a better understanding of the kinetics of biochemical reactions and of antibody-antigen interactions.

这项研究由化学学部分析和表面化学项目支持，将使用“终身分辨荧光检测圆二色性”（LRFDCD）研究手性分子与手性和非手性宿主之间的结合率，这种方法可以监测反应过程中光偏振的变化。光偏振和荧光寿命都与反应的速度和程度有关。要描述的系统包括免疫化学分析方法中的抗体-抗原相互作用、分子在有组织介质中的溶解作用以及手性溶质和色谱相在对映体分离中的相互作用。这项研究涉及客体分子与宿主空腔分子组分的相互作用。特别令人感兴趣的是客体腔和宿主腔的形状对这些相互作用的影响。手性和非手性宿主以及手性和非手性客体将使用一种名为“时间分辨荧光检测圆二色”的新技术进行研究。这些结果将适用于手性分子的色谱分离，以及更好地理解生化反应和抗体-抗原相互作用的动力学。