Lifetime-Resolved Fluorescence-Detected Circular Dichroism

这项研究得到了分析和表面的支持， 化学系的化学课程，将研究 手性分子和两个手性分子之间的缔合速率 和使用“寿命分辨荧光- 检测圆二色性”（LRFDCD），一种可以 监测反应期间光偏振的变化。 光偏振和荧光寿命都可以被 与反应的速度和程度有关。系统间 要表征的是抗体-抗原相互作用， 免疫化学分析方法，分子溶解 在有组织的介质中，以及手性溶质和 对映体分离中的色谱相。 %%% 本研究关注的是客人之间的相互作用 分子与宿主腔的分子成分。 的 特别感兴趣的是客人的形状的影响 以及宿主空腔对这些相互作用的影响。手性和 将研究非手性主体和手性及非手性客体 使用一种新技术，称为“时间分辨 荧光检测圆二色性。“结果将是 适用于手性化合物的色谱分离 分子和更好地了解动力学的 生物化学反应和抗体-抗原相互作用。

This research, supported by the Analytical and Surface Chemistry Program in the Chemistry Division, will study the rate of association between chiral molecules and both chiral and achiral hosts using "lifetime-resolved fluorescence- detected circular dichroism" (LRFDCD), a method which can monitor the change in light polarization during a reaction. Light polarization and fluorescence lifetime can both be related to the speed and extent of reaction. Among the systems to be characterized are antibody-antigen interactions in immunochemical methods of analysis, solubilization of molecules in organized media, and interactions between chiral solutes and chromatographic phases in enantiomeric separations. %%% This research is concerned with the interactions of guest molecules with the molecular components of host cavities. Of particular interest are the effects of the shapes of the guests and of the host cavities on these interactions. Chiral and achiral hosts and chiral and achiral guests will be studied using a new technique called "time-resolved fluorescence-detected circular dichroism." The results will be applicable to chromatomatographic separations of chiral molecules and to a better understanding of the kinetics of biochemical reactions and of antibody-antigen interactions.