Fundamental Gas-Phase Properties of Non-Protein Amino Acids and Non-Protein Amino Acid - Containing Peptides

非蛋白质氨基酸和含非蛋白质氨基酸的肽的基本气相性质

• 批准号: 0911244
• 负责人: John Poutsma
• 金额: $ 30万
• 依托单位: College of William and Mary
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0911244&HistoricalAwards=false
• 关键词: Fundamental; Gas; Phase; Properties; Non

In this award, funded by the Experimental Physical Chemistry Program, Professor Poutsma from College of William and Mary and his students will investigate amino acids and their gas phase ion chemistry. The first goal is to understand the subtle interplay between the structure of amino acids and their gas-phase energetic properties. The researchers will focus on the gas phase thermochemistry of so-called non-protein amino acids (NPAA), many of which are toxic to humans and other animals in part due to structural similarity to one or more of the twenty common protein amino acids (PAA). Absolute gas-phase thermochemical properties will be determined for naturally-occurring non-protein amino acids (NPAA) as compared to their PAA counterparts, both as isolated amino acids and when incorporated into small peptides.The second goal focuses on the effects of NPAA-substitution into small peptides which will be accomplished by studying fragmentation patterns of PAA peptides and their NPAA analogs. There is biological relevance to this project in that many NPAA can mis-incorporate into peptides and proteins. Detailed knowledge of any unusual fragmentation behavior for peptides containing NPAAs will be necessary in order to interpret mass spectra from both top-down and bottom-up proteomics experiments. The ultimate goal is to better understand the mechanisms for "selective" fragmentations in low-energy tandem mass spectrometry experiments that lead to incomplete sequence coverage for the peptide of interest. The main function of a primarily undergraduate chemistry department is the preparation of future generations of scientists. At the College of William and Mary, this charge is accomplished through both classroom teaching and undergraduate research. Since over eighty percent of the College's chemistry majors perform undergraduate research, over the three years of this award it is anticipated that 15-20 undergraduate students will perform research in Professor Poutsma's laboratory. Based on current demographics, approximately one-half of these students will be either female or a member of an underrepresented group.

在该奖项中，由实验物理化学计划资助，威廉和玛丽学院的POUTSMA教授和他的学生将研究氨基酸及其气相离子化学。第一个目标是了解氨基酸结构与其气相能量性能之间的微妙相互作用。研究人员将关注所谓的非蛋白氨基酸（NPAA）的气相热化学，其中许多对人类和其他动物有毒，部分原因是与二十种普通蛋白氨基酸（PAA）中的一个或多个结构相似。 Absolute gas-phase thermochemical properties will be determined for naturally-occurring non-protein amino acids (NPAA) as compared to their PAA counterparts, both as isolated amino acids and when incorporated into small peptides.The second goal focuses on the effects of NPAA-substitution into small peptides which will be accomplished by studying fragmentation patterns of PAA peptides and their NPAA analogs. 与该项目具有生物学相关性，因为许多NPAA可能会将其分解为肽和蛋白质。为了解释自上而下和自下而上的蛋白质组学实验的质谱，需要对含有NPAA的肽的任何异常碎片行为进行详细了解。 最终目标是更好地了解低能串联质谱实验中“选择性”碎片的机制，这些实验导致对感兴趣肽的不完全序列覆盖率。 主要是本科化学系的主要功能是准备后代的科学家。在威廉和玛丽学院，这项指控是通过课堂教学和本科研究来完成的。由于该学院的化学专业大满贯的本科研究中有超过80％的人在这项奖项的三年中，预计15-20名本科生将在POUTSMA教授的实验室中进行研究。 根据当前的人口统计，这些学生中的大约一半将是女性或代表性不足的成员。