SBIR Phase I: Metabolic Engineering for Clostridial Biotechnology

SBIR 第一阶段：梭菌生物技术的代谢工程

• 批准号: 0912547
• 负责人: BRYAN TRACY
• 金额: $ 9.95万
• 依托单位: ELCRITON, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0912547&HistoricalAwards=false
• 关键词: SBIR; Phase; Metabolic; Engineering; Clostridial

This Small Business Innovation Research Phase I project aims to develop platform clostridia strains suitable for industrial scale alcohol production from renewable feedstocks and also to improve metabolic engineering technologies for all clostridia. Clostridia are strictly anaerobic, endospore forming prokaryotes of major importance to cellulose degradation, human and animal health and physiology, anaerobic degradation of simple and complex carbohydrates. Obstacles for the industrial use of these organisms include the development of genetic and metabolic engineering tools and strategies that could lead to strains suitable for production of chemicals and fuels from renewable feedstocks. This project focuses on developing metabolic engineering strategies and strains of solventogenic clostridia for the production of chemicals and biofuels. Through novel approaches, this project aims to solve three important bioprocessing bottlenecks: 1) product formation characteristics, 2) product yield and selectivity, 3) and suitable characteristics for repeated fed-batch or continuous fermentations. Anticipated outcomes of this project are clostridia strains that overcome the aforementioned bioprocessing bottlenecks and improved metabolic engineering technologies that are applicable to all clostridia.Development of biorefinery and biofuel technologies has been on the scientific and technological agenda of our nation for over 35 years now but never quite with the urgency of the last 2-3 years. Oil supplies for producing chemicals and fuels are becoming increasingly limiting and unreliable. Moreover, use or combustion of non-renewable chemicals and fuels detrimentally impacts the climate of our planet. Biomass is a carbon-neutral renewable resource for producing chemicals and fuels and the basis for the biorefinery concept. Solventogenic, butyric-acid clostridia played a major industrial role in the production of acetone and butanol in the past. Metabolic engineering of solventogenic clostridia may lead to industrial processes for production of chemicals such as butyric acid, butanol, butanediol, propanol, and acetoin, and production of hydrogen. Some of these chemicals can serve as biofuels directly, while others can be used for chemical conversion to biofuels. A major advantage of these organisms is that they can directly ferment a large spectrum of simple and complex carbohydrates including lignocellulosics with minimal pretreatment. The commercial potential of metabolically engineered solventogenic clostridia is exceptional but remains largely unexplored. This project aims to capture and demonstrate part of this potential.This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).

该小型企业创新研究第一阶段项目旨在开发适用于利用可再生原料进行工业规模酒精生产的平台梭菌菌株，并改进所有梭菌的代谢工程技术。梭状芽胞杆菌是严格厌氧、形成内生孢子的原核生物，对纤维素降解、人类和动物健康和生理学、简单和复杂碳水化合物的厌氧降解具有重要意义。这些生物体工业化利用的障碍包括开发遗传和代谢工程工具和策略，这些工具和策略可能会产生适合从可再生原料生产化学品和燃料的菌株。该项目的重点是开发用于生产化学品和生物燃料的代谢工程策略和产溶剂梭菌菌株。 该项目旨在通过新颖的方法解决三个重要的生物加工瓶颈：1）产品形成特性，2）产品产量和选择性，3）以及适合重复补料分批或连续发酵的特性。该项目的预期成果是克服上述生物加工瓶颈的梭菌菌株，并改进适用于所有梭菌的代谢工程技术。生物炼制和生物燃料技术的发展已列入我国科技议程超过 35 年，但从未像过去 2-3 年那样紧迫。用于生产化学品和燃料的石油供应变得越来越有限且不可靠。此外，不可再生化学品和燃料的使用或燃烧会对地球的气候产生不利影响。生物质是一种碳中性可再生资源，用于生产化学品和燃料，也是生物炼制概念的基础。产溶剂丁酸梭菌过去在丙酮和丁醇的生产中发挥了重要的工业作用。 产溶剂梭菌的代谢工程可能导致生产丁酸、丁醇、丁二醇、丙醇和乙偶姻等化学品以及生产氢气的工业过程。其中一些化学品可以直接用作生物燃料，而另一些则可以用于化学转化为生物燃料。这些生物体的一个主要优点是它们可以直接发酵大量简单和复杂的碳水化合物，包括木质纤维素，只需最少的预处理。代谢工程产溶剂梭状芽胞杆菌的商业潜力非常巨大，但在很大程度上仍未得到开发。该项目旨在捕捉并展示这一潜力的一部分。该奖项由 2009 年美国复苏和再投资法案（公法 111-5）资助。