STTR Phase I: Integration of carbohydrate and gaseous fermentations for maximum C4 chemical yield

STTR 第一阶段：碳水化合物和气态发酵的整合，以获得最大的 C4 化学产量

• 批准号: 1346424
• 负责人: BRYAN TRACY
• 金额: $ 22.5万
• 依托单位: ELCRITON, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1346424&HistoricalAwards=false
• 关键词: STTR; Phase; Integration; carbohydrate; gaseous

This Small Business Technology Transfer Research Phase I project aims to enhance fermentation yield of four carbon (C4) chemicals by instating mixotrophic fermentation. In order to realize the most cost effective fermentation for commodity chemical and biofuel production, the process should achieve maximum conversion of feedstock. In carbohydrate fermentations, CO2 and H2 are commonly evolved, which negatively impacts yield of desired products. We hypothesize that mixotrophic fermentation can recapture that yield loss. We define mixotrophic fermentation as the simultaneous consumption of organic and inorganic substrates. Improvements in yield from mixotrophic fermentation can be very significant. Moreover, certain clostridial organisms in theory can perform such fermentation, but relatively little is known about this. Moreover, the genetic tools to manipulate these microorganisms are underdeveloped. Consequently, this Phase I STTR will develop a genetic toolbox for these microorganisms, interrogate their ability for simultaneous substrate utilization of both carbohydrate and gas, and demonstrate the potential to produce C4 chemicals from mixotrophic fermentation.The broader impact/commercial potential of this project is to develop renewable and domestic chemical production and transportation fuel technologies that are cheaper, greener and more sustainable. Project outcomes, have the potential to increase product yield 10 ? 50%, which greatly reduces production-operating expense. The potential to utilize CO2 in the fermentation, minimizes the carbon footprint of the process. Lastly, process sustainability is enhanced since a greater diversity of feedstocks can be concurrently used such as complex carbohydrates, five and six carbon sugar monomers, biodiesel waste, hydrolyzed biomass, syngas, waste gas, and activated methane molecules. Overall, the project has the commercial potential to improve the triple bottom line of many chemical companies. Furthermore, this project could significantly enhance scientific and technological understanding of microbial physiology and metabolism during gas and carbohydrate fermentation.

这个小企业技术转移研究第一阶段项目旨在通过启动混合营养发酵来提高四种碳（C4）化学品的发酵产量。为了实现用于商品化学品和生物燃料生产的最具成本效益的发酵，该方法应实现原料的最大转化。在碳水化合物发酵中，通常会产生CO2和H2，这对所需产物的产率产生负面影响。我们假设，混合营养发酵可以夺回产量损失。我们将混合营养发酵定义为同时消耗有机和无机底物。 来自混合营养发酵的产率的提高可以是非常显著的。 此外，理论上某些梭菌生物可以进行这种发酵，但对此知之甚少。 此外，操纵这些微生物的遗传工具还不发达。 因此，第一阶段STTR将为这些微生物开发一个遗传工具箱，询问它们同时利用碳水化合物和气体的能力，并展示从混合营养发酵生产C4化学品的潜力。该项目更广泛的影响/商业潜力是开发可再生和家用化学品生产和运输燃料技术，这些技术更便宜，更环保，更可持续。项目成果，有潜力提高产品产量10？50%，大大降低了生产运营费用。在发酵中利用CO2的潜力最大限度地减少了该过程的碳足迹。 最后，工艺可持续性得到增强，因为可以同时使用多种多样的原料，例如复合碳水化合物、五碳和六碳糖单体、生物柴油废物、水解生物质、合成气、废气和活化甲烷分子。总体而言，该项目具有提高许多化工企业三重底线的商业潜力。此外，该项目可以显着提高对气体和碳水化合物发酵过程中微生物生理学和代谢的科学和技术理解。