The proposal focuses on the investigation of the molecular basis of Myxococcus xanthus A-motility, using genetic and fluorescence microscopy techniques

该提案的重点是利用遗传和荧光显微镜技术研究黄色粘球菌 A 运动性的分子基础

• 批准号: 193253204
• 负责人: Dr. Christine Kaimer
• 金额: --
• 依托单位: Department of Molecular and Cell Biology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/193253204?language=en
• 关键词: proposal; focuses; investigation; molecular; basis

The coordinated movement of single cells is a prerequisite for social behavior patterns within the life cycle of the soil bacterium Myxococcus xanthus. Gliding movement on solid surfaces is mediated by social S-motility and adventurous A-motility. A-motility is likely powered by protein complexes that are distributed along the cell body. As a component of the A-motility complex, a molecular motor might exert force to a cellular structure, possibly the cytoskeleton, to push the cell forward. The proposed research project aims to gain further insight on the molecular mechanism of A-motility. Experiments focus on the identification and characterization of homologues to the Tol-Pal/TonB-systems that are involved in M. xanthus A motility. Mutational analysis as well as fluorescence microscopy, to determine the intracellular distribution of the proteins under various conditions, will be used to address their function. Moreover, the role of the cytoskeleton as a potential structure for attachment of a molecular motor will be investigated. For that purpose, fluorescence microscopy experiments will concentrate on the dynamics and rotation of cytoskeletal elements.

单细胞的协调运动是土壤细菌黄色粘球菌生活史中社会行为模式的先决条件。固体表面上的滑动运动是由社会S运动和冒险A运动所调节的。A-运动很可能是由分布在细胞体上的蛋白质复合体提供动力的。作为A-运动复合体的一个组成部分，分子马达可能会对细胞结构施加力，可能是细胞骨架，以推动细胞向前。这项拟议的研究项目旨在进一步深入了解A-运动的分子机制。实验的重点是鉴定和鉴定参与黄花莲A运动的Tol-Pal/TonB-系统的同系物。突变分析以及荧光显微镜，以确定不同条件下蛋白质在细胞内的分布，将被用来研究它们的功能。此外，还将研究细胞骨架作为连接分子马达的潜在结构的作用。为此，荧光显微镜实验将集中在细胞骨架元素的动力学和旋转上。