PFI: Transforming Environmental and Physiological Assessments Using Fish Erythrocyte Gene Expression to Measure Responses

PFI：利用鱼类红细胞基因表达来测量反应，转变环境和生理评估

• 批准号: 0917865
• 负责人: Ronald Hardy
• 金额: $ 60万
• 依托单位: Regents of the University of Idaho
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0917865&HistoricalAwards=false
• 关键词: PFI; Transforming; Environmental; Physiological; Assessments

This Partnerships for Innovation (PFI) project is a Type III (A:C) partnership between University of Idaho, an NSF PFI graduate (0090635), and Idaho State University, an institution new to the PFI Program (defined as one that has never been a PFI grantee) seeks to address the lack of modern techniques and protocols to assess physiological health of fish by development, validation, and calibration of a novel and potentially transformational product: the erythrocyte assay system for fish (EASY-F). This assay system will capitalize on the fact that fish erythrocytes are nucleated and thus have a complete genome to respond to environmental conditions and experimental manipulations. Assessment of the physiological health of fish requires sensitive, accurate tools that can provide rapid assessments and predictive information.Fish play crucial and multiple roles in human society; they are valued as essential components of the aquatic environment in the U.S.; they are considered biomarkers to assess the health of aquatic ecosystems, and they are important to the sport and recreation industry in the U.S. What unites the various roles of fish in the environment and human society is that they all depend on production of healthy fish, both in nature and in farms and hatcheries. Testing and development of the proposed EASY-F quantitative polymerase chain reaction (QPCR) mini array will have an impact on Native American interests in salmonids directly. Tribal Groups are co-managers of salmonid fisheries and each tribe operates its own fisheries department. Successful partnership with tribal stakeholders begins with research collaboration, technology sharing, and data exchange. Development and validations of the EASY-F system will rely in large part on the direct collaboration of tribal stakeholders. In turn, the use and commercialization of the EASY-F technology will directly affect tribal interests by providing additional opportunities to train tribal fisheries scientists, raise the level of technological expertise in tribal fisheries departments, and improve information exchange among stakeholders crucial to the co-management of the fisheries. Ultimately, technology transferred from this project has the potential to influence the management of fish populations affected by disease or other environmental stressors, populations under restoration, and captive populations being enhanced to increase productivity.Partners at the inception of the project are: Academic Institutions: University of Idaho (Moscow and Hagerman, ID) (lead institution), Idaho State University (Pocatello, ID), and University of Chile (Santiago de Chile; Private Sector Organizations: Cooke Aquaculture Inc. (Blacks Harbour, New Brunswick, Canada), Portneuf Regional Medical Center (Pocatello, Idaho); Federal Government Organizations: Agriculture Research Service, Department of Agriculture; and U.S. Fish and Wildlife Service (Bozeman Fish Technology Center, Bozeman, MT), Department of Interior; and Native American Organization: Columbia River Inter-Tribal Fish Commission (Portland, OR)..

该创新合作伙伴关系（PFI）项目是爱达荷州大学（NSF PFI毕业生（0090635））和爱达荷州州立大学（PFI计划的新机构（定义为从未获得PFI资助的机构））之间的III型（A：C）合作伙伴关系，旨在通过开发、验证和校准一种新的和潜在的转型产品来解决缺乏现代技术和协议的问题，以评估鱼类的生理健康：鱼类红细胞测定系统（EASY-F）。 该测定系统将利用鱼红细胞是有核的，因此具有完整的基因组以响应环境条件和实验操作的事实。鱼类生理健康的评估需要敏感，准确的工具，可以提供快速的评估和预测信息。鱼类在人类社会中发挥着至关重要的多重作用，它们被视为美国水生环境的重要组成部分。它们被认为是评估水生生态系统健康的生物标志物，并且它们对美国的体育和娱乐业很重要。将鱼类在环境和人类社会中的各种作用统一起来的是，它们都依赖于在自然界以及农场和孵化场中健康鱼类的生产。EASY-F定量聚合酶链反应（QPCR）微型阵列的测试和开发将直接影响美洲原住民对鲑鱼的兴趣。 部落团体是鲑鱼渔业的共同管理者，每个部落都有自己的渔业部门。 与部落利益相关者的成功伙伴关系始于研究合作，技术共享和数据交换。 EASY-F系统的开发和验证将在很大程度上依赖于部落利益相关者的直接合作。 反过来，EASY-F技术的使用和商业化将直接影响部落的利益，因为它提供了更多的机会来培训部落渔业科学家，提高部落渔业部门的技术专门知识水平，并改善对渔业共同管理至关重要的利益攸关方之间的信息交流。 最终，从该项目转让的技术有可能影响受疾病或其他环境压力影响的鱼类种群的管理，恢复中的种群，以及增加圈养种群以提高生产力。爱达荷州大学（莫斯科和印第安纳州哈格曼）（牵头机构）、爱达荷州州立大学（印第安纳州波卡泰洛）和智利大学（智利圣地亚哥）;私营部门组织：库克水产公司。（布拉克斯港，新不伦瑞克，Canada），波特纳夫Regional Medical Center（波卡泰洛，爱达荷州）;联邦政府组织：农业部农业研究服务;和美国渔业和野生动物服务（Bozeman Fish Technology Center，Bozeman，MT），内政部;和美洲原住民组织：哥伦比亚河部落间渔业委员会（波特兰，OR）。