Evolutionary Studies of the Heat Shock Response and Thermotolerance in Boechera: Transcriptomics, Physiology and Gene Evolution

Boechera 热激反应和耐热性的进化研究：转录组学、生理学和基因进化

• 批准号: 0920611
• 负责人: Elizabeth Waters
• 金额: $ 58.34万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920611&HistoricalAwards=false
• 关键词: Evolutionary; Studies; Heat; Shock; Response

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)." This project will study four species of Rockcress plants (Brassicaceae family, Boechera genus). These plants are native to California. Two of these species live in deserts and two are found either on the coast or in the mountains. These plants differ in their ability to survive and grow when exposed to high temperatures. Plants cannot move away or avoid high temperature stress and therefore, the ability to survive high temperature stress is a very important trait for plants. While heat stress has been studied in some crop species very little is known of the differences in tolerance to stress among wild plant species. This project will address this important but yet under studied question. In order to obtain a full understanding of how Rockcress plants respond to high temperature stress studies will be conducted that will examine rates of photosynthesis, and overall plant growth. In addition, the gene expression patterns of both the heat-tolerant and heat-sensitive plants will be examined both before and after stress. By examining the patterns of gene expression among species that respond differently to stress the researchers will identify novel genes and proteins that provide tolerance to heat stress. In addition to deepening our understanding of how wild plants adapt to stress this research will also provide information that will assist in understanding how crop species may acquire tolerance to heat stress. During this project a wide range of students (both undergraduate and graduate) will be trained in modern scientific methods. Preference will be given to students that are the first in their families to attain a university degree and/or are members of groups that are under-represented in science. In addition, a post-doctoral fellow will be trained during this project and will be provided with mentoring to facilitate his or her independent research career.

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。“本计划将研究四种岩芥属植物（岩芥科，Boechera属）。这些植物原产于加州。其中两种生活在沙漠中，两种在海岸或山区。这些植物在高温下的生存和生长能力不同。植物不能避开或避开高温胁迫，因此，在高温胁迫下生存的能力是植物的一个非常重要的性状。虽然在一些作物物种中研究了热胁迫，但对野生植物物种之间的胁迫耐受性差异知之甚少。本项目将解决这一重要但尚未研究的问题。为了充分了解岩芥植物如何应对高温胁迫，将进行研究，研究光合作用速率和整体植物生长。此外，还将检测耐热植物和热敏感植物在胁迫前后的基因表达模式。通过研究对压力反应不同的物种之间的基因表达模式，研究人员将确定新的基因和蛋白质，提供对热应激的耐受性。除了加深我们对野生植物如何适应胁迫的理解外，这项研究还将提供有助于理解作物物种如何获得对热胁迫的耐受性的信息。在这个项目中，各种各样的学生（包括本科生和研究生）将接受现代科学方法的培训。优先考虑家庭中第一个获得大学学位的学生和/或科学代表性不足的群体的成员。此外，一名博士后研究员将在本项目期间接受培训，并将得到指导，以促进他或她的独立研究生涯。