Regulation of meristem function by AIL proteins

AIL 蛋白对分生组织功能的调节

• 批准号: 0922367
• 负责人: Beth Krizek
• 金额: $ 42.04万
• 依托单位: University South Carolina Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922367&HistoricalAwards=false
• 关键词: Regulation; meristem; function; AIL; proteins

Beth A. KrizekProposal Number: IOS-0922367Proposal Title: Regulation of meristem function by AIL proteinsThis award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).The unique ability of plants to initiate organs throughout their lifespan results from the activity of meristems. The shoot apical meristem, a group of cells at the apex of the plant, provides cells for the initiation of lateral organs such as leaves and flowers while maintaining a central core of stem cells that remain undifferentiated. The shoot apical meristem is highly organized and yet dynamic, responding to local signals and those originating from more distant parts of the plant. Gradients of plant hormones within the meristem appear to be important in balancing stem cell renewal with organ initiation. But it is not clear how these hormone gradients are generated and how they are translated into different cellular behaviors. This project will investigate the hormonal regulation of shoot apical meristem function in the model plant Arabidopsis thaliana. The role of three related AINTEGUMENTA-like (AIL) proteins in mediating the antagonistic behavior of two plant hormones (auxin and cytokinin) within the meristem will be investigated by genetic, molecular and live imaging approaches. The expected results from this work are an integration of these AIL genes into a genetic framework for meristem function, molecular information on how these proteins regulate hormone levels/responsiveness, and a spatiotemporal map of the dynamic interactions between the expression of these proteins and hormone gradients within the meristem. Because the shoot apical meristem shapes the overall architecture of plants, this work has broad implication for the design of food and biofuel crops with improved yields and the production of new varieties of ornamental plants. The project will provide training opportunities for students, a postdoctoral fellow and a K-12 teacher.

Beth A. Krizekpropopals编号：ios-0922367港口标题：AIL Proteins奖对分生生物功能的调节，这是根据2009年《美国复苏和再投资法》（公共法律111-5）资助的。植物在Meristems的活动中启动器官的启动器官的独特能力。芽的顶端分生组织是植物顶点处的一组细胞，为启动侧侧器官（例如叶子和花朵）的细胞提供了细胞，同时保持了保持未分化的干细胞中心核心。芽的顶端分生组织具有高度组织且动态的，响应局部信号以及源自植物更遥远的部分的信号。分生组织内植物激素的梯度似乎在平衡干细胞更新与器官启动方面很重要。但是，尚不清楚如何产生这些激素梯度以及如何将它们转化为不同的细胞行为。该项目将研究模型植物拟南芥中芽顶分生组织功能的激素调节。三种相关的Aintegumenta样蛋白（AIL）蛋白在分生分生组织中两种植物激素（生长素和细胞分裂素）的拮抗行为中的作用将通过遗传，分子和实时成像方法研究。这项工作的预期结果是将这些AIL基因整合到分生组织功能的遗传框架中，这些蛋白质如何调节激素水平/反应性的分子信息以及这些蛋白质表达和激素梯度之间动态相互作用的时空图。由于芽的顶端分生组织塑造了植物的整体建筑，因此这项工作对以提高产量和新品种的装饰植物生产的食物和生物燃料作物的设计具有广泛的影响。该项目将为学生，博士后研究员和K-12老师提供培训机会。