International Collaboration in Chemistry: Spatially Resolved Measurements of Cellular Releasates

国际化学合作：细胞释放物的空间分辨测量

• 批准号: 0924320
• 负责人: Daniel Chiu
• 金额: $ 30万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0924320&HistoricalAwards=false
• 关键词: International; Collaboration; Chemistry; Spatially; Resolved

Professor Dan Chiu at the University of Washington is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry with co-funding from the Office of International Science and Engineering for an International Collaboration in Chemistry (ICC) seeking to develop new methodology for monitoring the spatiotemporal dynamics of cellular releasates. Through the International Collaboration in Chemistry Program, the work includes collaboration with Professor David McGloin at the University of Dundee (UK); Dr. McGloin's work is supported by the Engineering and Physical Sciences Research Council (EPSRC) of the UK.The approach is based on holographic optical trapping, sensitive microfluidic manipulation, and single-molecule assays. It targets orders of magnitude improvements in spatial resolution and sensitivity over current techniques, enabling progress toward answering biological questions that are currently inaccessible with existing methods. The technology pushes the current limits in holographic optical trapping and imaging, develops new techniques in microfluidic manipulation, and expands current capabilities in single-molecule assays. Applications address the spatial and temporal dynamics of cell secretion and cell-cell communication, seeking a new level of understanding on the functioning of immune cells.In addition to the technical impacts, the project provides broader impact through a unique multidisciplinary international training experience for graduate students via bidirectional visits.

华盛顿大学的Dan Chiu教授得到了化学系分析和表面化学项目的支持，并与国际科学和工程办公室共同资助了一项国际化学合作(ICC)，旨在开发新的方法来监测细胞释放物的时空动力学。通过国际化学合作计划，这项工作包括与英国邓迪大学的David McGloin教授合作；McGloin博士的工作得到了英国工程和物理科学研究理事会(EPSRC)的支持。该方法基于全息光学捕获、灵敏的微流体操纵和单分子分析。它的目标是在空间分辨率和灵敏度方面比目前的技术提高几个数量级，使回答目前无法用现有方法获得的生物学问题取得进展。这项技术突破了目前全息光学捕获和成像的极限，开发了微流控操作的新技术，并扩展了单分子分析的现有能力。应用程序解决了细胞分泌和细胞间交流的时空动态，寻求对免疫细胞功能的新水平的理解。除了技术影响外，该项目通过双向访问为研究生提供独特的多学科国际培训体验，提供更广泛的影响。