Investigation of chemical reactions in femtoliter-volume droplets

飞升体积液滴中化学反应的研究

• 批准号: 0844688
• 负责人: Daniel Chiu
• 金额: $ 46.96万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-15 至 2013-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0844688&HistoricalAwards=false
• 关键词: Investigation; chemical; reactions; femtoliter; volume

Professor Daniel T. Chiu of the University of Washington is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to study chemical reactions in femtoliter-volume droplets. Surfactants with varying concentrations, immiscible oils, and interior solute ionic strength will be varied and tested to determine their exact effects on droplets. The experimental information obtained will be used to synthesize a model to allow prediction of droplet confined chemical reactions, and how they are affected by the choice of surfactant and immiscible oil as well as the concentration and ionic strength of the solutes contained within the droplet. While droplet microfluidics are beginning to find broad use in diverse areas, such as biological assays, protein crystallization, DNA sequencing, and chemical synthesis, the fundamentals of chemistry in droplets, in particular, how the high surface-to-volume ratio environment affects the chemistry, is still relatively unexplored. Students will be trained in interfacial chemistry as well as microfluidics and cutting-edge optical techniques for manipulating and analyzing the contents of droplets. The predictive model and associated software will be made available on the PI's laboratory website.

华盛顿大学的 Daniel T. Chiu 教授在化学系分析和表面化学项目的支持下研究飞升体积液滴中的化学反应。 具有不同浓度的表面活性剂、不混溶的油和内部溶质离子强度将被改变并进行测试，以确定它们对液滴的确切影响。 获得的实验信息将用于合成一个模型，以预测液滴限制的化学反应，以及它们如何受到表面活性剂和不混溶油的选择以及液滴内溶质的浓度和离子强度的影响。 虽然液滴微流体开始在生物测定、蛋白质结晶、DNA测序和化学合成等不同领域得到广泛应用，但液滴化学的基本原理，特别是高表面积与体积比的环境如何影响化学，仍然相对未经探索。 学生将接受界面化学以及微流体和尖端光学技术方面的培训，以操纵和分析液滴的内容。 预测模型和相关软件将在 PI 实验室网站上提供。