Hybrid Bionanomechanical Systems for Single Molecule Detection

用于单分子检测的混合生物纳米机械系统

• 批准号: 0925417
• 负责人: Cagri Savran
• 金额: $ 27万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0925417&HistoricalAwards=false
• 关键词: Hybrid; Bionanomechanical; Systems; Single; Molecule

Hybrid Bio-Nanomechanical Systems for Single Molecule DetectionCagri Savran, Purdue UniversityThis award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).AbstractThe objective of this research is to develop a novel nanomechanical sensor with single molecule detection sensitivity. The approach is to form a hybrid cantilever with a functional virus at its tip. The cantilever/virus hybrid structure will be used as a fishing rod to reel in the DNA, whereby the other end of the DNA will capture a target entity from a sample by means of an antibody. The packaging and reeling of the DNA upon the capture of target analyte will be detected using magnetic actuation via a magnetic bead that binds to the captured molecule.Intellectual merit: The developed system will be the first sensor that uses a virus activity to achieve: single molecule sensitivity, efficient target capture (target-capturing antibody will not be immobilized on a surface, rather it will be extended into solution via DNA) and negligible nonspecific binding (only the tip of the cantilever where the virus is located will be in contact with the solution) and hence will finally allow nanomechanical sensors to be useful in biological research and medicine. Broad impact: This project has the potential to result in biosensors that are extremely sensitive which can penetrate into clinical and biological research laboratories and serve to numerous goals that range from disease biomarker detection to understanding of protein-protein interactions at the single molecule level. Also, as a part of this proposal, the laboratory portion of an undergraduate course will be modified to have MEMS/NEMS devices, and hence will educate undergraduates about this new field for years to come.

用于单分子检测的混合生物纳米机械系统Cagri Savran，普渡大学该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。该方法是在其尖端形成具有功能性病毒的混合悬臂。悬臂/病毒混合结构将被用作钓鱼杆以卷起DNA，由此DNA的另一端将通过抗体从样品中捕获目标实体。在捕获目标分析物时，DNA的包装和卷绕将通过与捕获分子结合的磁珠使用磁致动来检测。智力价值：开发的系统将是第一个使用病毒活动来实现以下目标的传感器：单分子灵敏度，有效的目标捕获（靶捕获抗体不会固定在表面上，相反，它将通过DNA延伸到溶液中）和可忽略的非特异性结合（只有病毒所在的悬臂尖端与溶液接触），因此最终将使纳米机械传感器在生物研究和医学中发挥作用。广泛影响：该项目有可能产生非常敏感的生物传感器，可以渗透到临床和生物研究实验室，并用于从疾病生物标志物检测到单分子水平上蛋白质-蛋白质相互作用的理解等众多目标。此外，作为该提案的一部分，本科课程的实验室部分将被修改为具有MEMS/NEMS设备，因此将在未来几年内教育本科生这一新领域。