SBIR Phase I: Development and Characterization of a Bioaugmentation Culture to Remediate Chlorinated Ethenes in Low pH Ground Water

SBIR第一阶段项目将开发一种强大的商业耐酸厌氧细菌Dehalococcoides（Dhc）培养物，用于原位处理受四氯乙烯（PCE）和三氯乙烯（TCE）污染的地下水。 项目小组将在酸性污染场地寻找具有耐酸能力的天然Dhc菌株。 最有前途的菌株将结合起来，创造一个商业脱氯文化，拟议项目的更广泛/商业影响将是部署一个生物修复解决方案，以解决和解决许多现有的酸引起的补救失败，从而提高现有的原位生物处理技术的有效性，作为一个具有成本效益和成功的补救选择，以消除地下水中的四氯乙烯和三氯乙烯。

This SBIR Phase I project will develop a robust commercial acid-tolerant culture of the anaerobic bacterium Dehalococcoides (Dhc) for in-situ treatment of groundwater contaminated with tetrachloroethene (PCE) and trichloroethene (TCE). The project team will conduct a search of acidic contaminated sites for natural Dhc strains having acid tolerance. The most promising strains will be combined to create a commercial dechlorinating culture.The broader/commercial impact of the proposed project will be the deployment of a bioremediation solution to address and solve many existing acid-caused remediation failures and thereby increase the effectiveness of existing in-situ biological treatment techniques as a cost-effective and successful remedial option to eliminate PCE and TCE from groundwater.