SynBERC BioFAB Facility

SynBERC BioFAB 设施

• 批准号: 0946510
• 负责人: Adam Arkin
• 金额: $ 140万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0946510&HistoricalAwards=false
• 关键词: SynBERC; BioFAB; Facility

ABSTRACT0946510, Arkin, University of California-BerkeleyThe primary purpose of this bio-fabrication facility (BioFab) is to ensure that all of the driving testbed applications of the Synthetic Biology Engineering Research Center (SynBERC) that are both existing and future have rapid access to high-quality standard biological parts as well as DNA synthesis and part assembly services. Two critical secondary purposes are: (1) the development and testing of the best organizational architecture for operating and scaling a production facility that is capable of producing many standard biological parts and that supports broader community participation across both academia and industry, and (2) ensuring that all foundational SynBERC-developed technologies, from rapid genome-scale reengineering of chassis to function composition standards for devices are broadly accessible and rapidly translated into SynBERC testbeds and to industry. Practically, the facility will pursue three specific projects at launch. Specifically, the BioFab staff will 1) rapidly prototype SynBERC-specified engineered genetic systems to achieve testbed-driven objectives, 2) design, construct, and test a collection of 6,000 new BioBrick parts for controlling replication, transcription, RNA processing and degradation, translation, and protein degradation in E.coli and S. cerevisiae, and 3) work in partnership with industrial and academic partners to develop improved tools for supporting the design, construction, and characterization/testing of engineered genetic systems assembled from standard biological parts. Intellectual merit: The successful development, launch, and operation of the world?s first design and build facility for making high-quality standard biological parts will mark an important transition in the development of biology as a substrate for engineering. Within academia and industry, the BioFab will mark a transition from individual ?crafts? based production of genetic reagents to a more mature engineering technology platform. In turn, this will make the engineering of biology easier and more predictable, with potential applications in energy, human health, information processing, and more. Broader impacts: This first-of-its-kind facility has significant broader impacts for academic research, education, industry, and globally. The BioFab facility will accelerate the adoption and development of biological standards while extending the SynBERC?s cutting-edge research to the broader industrial and academic communities. This professionally staffed and operated parts engineering facility will serve as an important resource for industry and academia for defining and promulgating standards, from technical to professional practice. Within education, engineering students will have access for the first time to improved collections of biological parts for controlling basic but essential cellular processes, allowing them to focus less on technical issues and more on identifying ways to engineer biology to solve the world?s problems.

这个生物制造工厂(BIOFAB)的主要目的是确保合成生物工程研究中心(SynBERC)现有和未来的所有驾驶试验台应用程序都能快速获得高质量的标准生物部件以及DNA合成和部件组装服务。两个关键的次要目标是：(1)开发和测试运营和扩大生产设施的最佳组织架构，该生产设施能够生产许多标准生物部件，并支持学术界和业界更广泛的社区参与，以及(2)确保SynBERC开发的所有基础技术，从机箱的快速基因组规模重组到设备的功能组成标准，都可广泛使用，并迅速转化为SynBERC试验床和工业。实际上，该设施将在启动时进行三个具体项目。具体地说，BIOFAB的工作人员将1)快速制作SynBERC指定的工程基因系统的原型，以实现试验台驱动的目标；2)设计、构建和测试6,000个新的BioBrick部件，用于控制大肠杆菌和酿酒酵母中的复制、转录、RNA加工和降解、翻译以及蛋白质降解；3)与工业和学术合作伙伴合作，开发改进的工具，用于支持由标准生物部件组装的工程基因系统的设计、构建和表征/测试。智力优势：世界的成功开发、发射和运营？S首先设计和建造了制造高质量标准生物部件的设施，这将标志着生物学作为工程底物发展的一个重要转变。在学术界和工业界，BIOFAB将标志着从个人手工艺？以基因试剂生产为基础的更成熟的工程技术平台。反过来，这将使生物工程变得更容易和更可预测，在能源、人类健康、信息处理等领域具有潜在的应用。更广泛的影响：这一首创的设施对学术研究、教育、工业和全球产生了重大的更广泛的影响。该机构将加速生物标准的采用和发展，同时将SynBERC？S的尖端研究扩展到更广泛的工业界和学术界。这个配备专业人员和运营的零部件工程设施将成为工业界和学术界定义和颁布从技术到专业实践的标准的重要资源。在教育领域，工科学生将首次有机会获得用于控制基本但必不可少的细胞过程的改进的生物部件集合，使他们能够更少地关注技术问题，而更多地关注如何通过工程生物学来解决世界上的问题--S问题。