SynBERC BioFAB Facility

摘要0946510，Arkin，加州大学伯克利分校这个生物制造设施（BioFab）的主要目的是确保合成生物学工程研究中心（SynBERC）现有和未来的所有驱动试验台应用都能快速获得高质量的标准生物部件以及DNA合成和部件组装服务。两个重要的次要目的是：（1）开发和测试最佳组织架构，用于运营和扩大生产设施，该设施能够生产许多标准生物部件，并支持学术界和工业界更广泛的社区参与，以及（2）确保所有基础SynBREC开发的技术，从快速基因组规模的底盘再设计到设备的功能组合标准，都可以广泛使用，并迅速转化为SynBERC测试平台和行业。实际上，该基金将在启动时开展三个具体项目。具体而言，BioFab的工作人员将1）快速原型化SynBERC指定的工程遗传系统，以实现测试台驱动的目标，2）设计，构建和测试6,000个新的BioBrick部件，用于控制大肠杆菌和S.coli中的复制，转录，RNA加工和降解，翻译和蛋白质降解。酿酒酵母，和3）与工业和学术合作伙伴合作，开发改进的工具，以支持设计，构建和表征/测试从标准生物部件组装的工程遗传系统。智力价值：世界的成功开发、发射和运作？的第一个设计和建造设施，使高质量的标准生物部件将标志着一个重要的转变，在生物学的发展作为一个基板的工程。在学术界和工业界，BioFab将标志着从个人？工艺品？以基因试剂生产为基础，建立了较为成熟的工程技术平台。反过来，这将使生物工程更容易和更可预测，在能源，人类健康，信息处理等方面具有潜在的应用。更广泛的影响：这是同类设施中的第一个，对学术研究，教育，工业和全球产生了重大的广泛影响。BioFab设施将加速生物标准的采用和开发，同时扩展SynBERC？的前沿研究，以更广泛的工业和学术界。 这个专业人员和操作的零件工程设施将作为行业和学术界的重要资源，用于定义和颁布从技术到专业实践的标准。在教育中，工程专业的学生将首次获得用于控制基本但必不可少的细胞过程的生物部件的改进集合，使他们能够更少地关注技术问题，而更多地关注如何设计生物学来解决世界？的问题。

ABSTRACT0946510, Arkin, University of California-BerkeleyThe primary purpose of this bio-fabrication facility (BioFab) is to ensure that all of the driving testbed applications of the Synthetic Biology Engineering Research Center (SynBERC) that are both existing and future have rapid access to high-quality standard biological parts as well as DNA synthesis and part assembly services. Two critical secondary purposes are: (1) the development and testing of the best organizational architecture for operating and scaling a production facility that is capable of producing many standard biological parts and that supports broader community participation across both academia and industry, and (2) ensuring that all foundational SynBERC-developed technologies, from rapid genome-scale reengineering of chassis to function composition standards for devices are broadly accessible and rapidly translated into SynBERC testbeds and to industry. Practically, the facility will pursue three specific projects at launch. Specifically, the BioFab staff will 1) rapidly prototype SynBERC-specified engineered genetic systems to achieve testbed-driven objectives, 2) design, construct, and test a collection of 6,000 new BioBrick parts for controlling replication, transcription, RNA processing and degradation, translation, and protein degradation in E.coli and S. cerevisiae, and 3) work in partnership with industrial and academic partners to develop improved tools for supporting the design, construction, and characterization/testing of engineered genetic systems assembled from standard biological parts. Intellectual merit: The successful development, launch, and operation of the world?s first design and build facility for making high-quality standard biological parts will mark an important transition in the development of biology as a substrate for engineering. Within academia and industry, the BioFab will mark a transition from individual ?crafts? based production of genetic reagents to a more mature engineering technology platform. In turn, this will make the engineering of biology easier and more predictable, with potential applications in energy, human health, information processing, and more. Broader impacts: This first-of-its-kind facility has significant broader impacts for academic research, education, industry, and globally. The BioFab facility will accelerate the adoption and development of biological standards while extending the SynBERC?s cutting-edge research to the broader industrial and academic communities. This professionally staffed and operated parts engineering facility will serve as an important resource for industry and academia for defining and promulgating standards, from technical to professional practice. Within education, engineering students will have access for the first time to improved collections of biological parts for controlling basic but essential cellular processes, allowing them to focus less on technical issues and more on identifying ways to engineer biology to solve the world?s problems.