Collaborative Research: ATOL: A Phylogenetic and Genomic Investigation of the Algal Heterokont Tree

合作研究：ATOL：藻类异质树的系统发育和基因组研究

• 批准号: 0949211
• 负责人: Robert Andersen
• 金额: $ 63.67万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-16 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0949211&HistoricalAwards=false
• 关键词: Collaborative; Research; ATOL; Phylogenetic; Genomic

An award has been made to Robert A. Andersen (Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine), Rose Ann Cattolico and Gabrielle Rocap (University of Washington, Seattle, Washington), Robert Jansen, Elizabeth Ruck and Edward Theriot (University of Texas, Austin Texas), Matt Julius (St. Cloud State University, St. Cloud, Minnesota), Stefano Draisma (National Herbarium and Leiden University, Leiden, The Netherlands) and Hiroshi Kawai (Kobe University, Kobe, Japan) to determine the relationships among the heterokont classes by generating two large molecular data sets. Currently, 14 taxonomic classes of heterokont algae are recognized, and they include over 100,000 described species (as many as one million total species has been estimated by experts). Common members include brown seaweeds (e.g., kelp) and the diatoms. Remarkably, despite two centuries of light microscopic study, 50 years of electron microscopic study, and 20 years of molecular investigations, the phylogenetic relationships among the 14 classes remains unknown. DNA sequences of seven nuclear, mitochondrial, and chloroplast genes from 270 heterokont algal species and 30 nonalgal relatives will be obtained and, entire chloroplast genomes from 30 species will be sequenced. The genome sequences will provide important new data for understanding chloroplasts and their origin(s), especially since knowledge in this field is greatly biased toward land plants. Algae serve as the base of the food chain for all organisms that dwell in the ocean and account for approximately half of the global photosynthesis, and therefore they provide approximately 50% of the oxygen we breathe. Members of the heterokont algae vary in morphology from simple unicells to highly complex seaweeds. Brown algae are the principal elements of seaweed beds and these macroalgae have high economic values as food and biomass resources. A number of heterokont algae are harmful to marine life. For example, some algae produce toxins that when concentrated in shellfish kill humans, while other species of heterokonts are well-known fish killers. Conversely, other heterokonts serve as a human food source, they are used in industrial processes and they have found new recognition in the nanotechnology industry. The closest relatives to the heterokont algae are the pseudofungi, which include many plant pathogens, such as the fungus that caused the Irish potato famine, and various molds and mildews. Graduate and undergraduate students and postdoctoral fellows from diverse cultural and ethnic backgrounds will be supported and trained in a wide diversity of fields, including evolution, genomics, molecular biology, computational biology, and plant biology. The research will be integrated with K-12 educators, public museums and parks using already established programs at several of the participating institutions. A Deep Brown web site already has been established, and it will be greatly expanded as part of this project.

一个奖项被授予罗伯特·A。安徒生（毕格罗海洋科学实验室，西布斯贝港，缅因州），罗斯安卡托利科和加布里埃尔罗卡普（华盛顿大学，西雅图，华盛顿），罗伯特詹森，伊丽莎白鲁克和爱德华塞里奥特（德克萨斯大学奥斯汀分校），马特·朱利叶斯（圣克劳德州立大学，圣克劳德，明尼苏达州），斯特凡诺·德雷斯马（National Herbarium and Leiden University，Leiden，The Netherlands）和Hiroshi Kawai（科比大学，科比，日本）通过生成两个大的分子数据集来确定异鞭毛类之间的关系。 目前，异鞭毛藻类的14个分类类别被确认，并且它们包括超过100，000个描述的物种（专家估计多达一百万个物种）。 常见的成员包括棕色海藻（例如，海藻）和硅藻。 值得注意的是，尽管经过了两个世纪的光学显微镜研究、50年的电子显微镜研究和20年的分子研究，这14个纲之间的系统发育关系仍然是未知的。将获得来自270种异鞭毛藻和30种非藻类亲属的7个核、线粒体和叶绿体基因的DNA序列，并对30种异鞭毛藻的整个叶绿体基因组进行测序。基因组序列将为了解叶绿体及其起源提供重要的新数据，特别是因为该领域的知识在很大程度上偏向陆地植物。藻类是所有生活在海洋中的生物的食物链的基础，占全球光合作用的大约一半，因此它们提供了我们呼吸的大约50%的氧气。 异形体藻类的成员在形态上从简单的单细胞到高度复杂的海藻变化。褐藻是海藻床的主要成分，这些大型海藻作为食物和生物质资源具有很高的经济价值。 许多异鞭毛藻对海洋生物有害。例如，一些藻类产生毒素，当集中在贝类中时会杀死人类，而其他种类的异鞭毛类是众所周知的鱼类杀手。相反，其他heterokonts作为人类的食物来源，它们被用于工业过程，它们在纳米技术行业中得到了新的认可。与异鞭毛藻最接近的亲戚是假真菌，其中包括许多植物病原体，例如导致爱尔兰马铃薯饥荒的真菌，以及各种霉菌和霉菌。来自不同文化和种族背景的研究生和本科生以及博士后研究员将在广泛的领域获得支持和培训，包括进化，基因组学，分子生物学，计算生物学和植物生物学。该研究将与K-12教育工作者，公共博物馆和公园整合，使用几个参与机构已经建立的项目。已经建立了一个深棕色网站，作为该项目的一部分，该网站将大大扩大。