Dynamics Of Strand-Crossover Formation In Cadherin

钙粘蛋白链交叉形成的动力学

• 批准号: 0950494
• 负责人: Susan Pedigo
• 金额: $ 56.08万
• 依托单位: University of Mississippi
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0950494&HistoricalAwards=false
• 关键词: Dynamics; Strand; Crossover; Formation; Cadherin

Lay AbstractMCB-0950494Dynamics of Strand-Crossover Formation in CadherinPedigo, SusanIntellectual merit: Cadherins are proteins that protrude from the surface of cells and interact with identical proteins on neighboring cells to form adhesions between cells. These protein-protein interactions between cadherins on opposing cells lead to solid tissue formation. Such interactions are critical during normal processes such as development and tissue maintenance, and also during abnormal processes such a cancer. Neural-cadherins are found primarily in brain tissue. Epithelial cadherins are found primarily in epithelial cells. These two proteins are very close relatives, but must have some difference that makes them suited for their particular tissue type. The goal of this project is to investigate the differences in the protein-protein interactions in neural- and epithelial-cadherins that lead to their segregation into particular tissues. Recently, this laboratory has observed a very striking difference between these two proteins. Preliminary results indicate that the protein-protein interaction between neural-cadherins is very slow to dissociate while that between epithelial-cadherins dissociates quickly. This difference in assembly dynamics is so striking that it may explain the segregation of these two proteins into distinct physiological niches. Experiments proposed here elaborate this basic observation by studying the details of how the interactions occur and the factors that contribute to the difference in the dynamics of the interactions. The project will investigate the protein-protein interactions in solution and in single-molecule studies using fluorescence techniques. Broader impacts. The experiments proposed here require the collaborative efforts and the diverse skills of the Pedigo and Hammer laboratories. The laboratories will use a wide range of experimental approaches that will expose students to diverse disciplines in chemistry. Training undergraduate and graduate students is critical to the research efforts of this project. The project will involve three graduate students as the core of the research team and a number of undergraduates through the Honors College and summer research internship programs at this university. The plan is to continue involvement in local and regional science education through visits of groups to the university, visits of students and faculty to elementary schools and participation in the science fairs.

Lay AbstrinMCB-0950494钙粘附素的链-交叉形成动力学智力优点：钙粘附素是从细胞表面伸出的蛋白质，并与相邻细胞上的相同蛋白质相互作用，形成细胞之间的粘连。相反细胞上的钙粘附素之间的这些蛋白质-蛋白质相互作用导致固体组织的形成。这种相互作用在发育和组织维持等正常过程中至关重要，在癌症等异常过程中也是如此。神经钙粘附素主要存在于脑组织中。上皮钙粘附素主要存在于上皮细胞。这两种蛋白质是非常亲密的亲缘关系，但一定有一些差异，使它们适合于他们特定的组织类型。这个项目的目标是研究导致神经和上皮钙粘附素分离到特定组织中的蛋白质-蛋白质相互作用的差异。最近，这个实验室观察到了这两种蛋白质之间非常显著的差异。初步结果表明，神经-钙粘附素之间的蛋白质-蛋白质相互作用解离很慢，而上皮-钙粘附素之间的蛋白质-蛋白质相互作用解离很快。这种组装动力学上的差异是如此显著，以至于它可以解释这两种蛋白质为什么分离成不同的生理生态位。这里提出的实验通过研究相互作用如何发生的细节以及导致相互作用动力学差异的因素来详细说明这一基本观察。该项目将使用荧光技术研究蛋白质在溶液中和单分子研究中的相互作用。更广泛的影响。这里提出的实验需要儿科和锤子实验室的合作努力和不同的技能。这些实验室将使用广泛的实验方法，使学生接触到化学中的不同学科。培养本科生和研究生对该项目的研究工作至关重要。该项目将包括三名研究生作为研究团队的核心，以及一些本科生通过荣誉学院和这所大学的暑期研究实习计划。该计划将通过团体访问大学、学生和教职员工访问小学以及参加科学博览会，继续参与当地和地区的科学教育。