Exploring the Biochemical Links between Arabidopsis Roots and Natural Soil Microbial Communities

探索拟南芥根与自然土壤微生物群落之间的生化联系

• 批准号: 0950857
• 负责人: Jorge Vivanco
• 金额: $ 74.07万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0950857&HistoricalAwards=false
• 关键词: Exploring; Biochemical; Links; between; Arabidopsis

Intellectual Merit:Plants initiate interactions with soil microbiota through root exudation. Thus, manipulating root exudation of plants presents a unique opportunity to influence soil microbial communities in a manner that will ultimately benefit plant/crop health. This project will provide such tools by characterizing a mutant (Atpdr2) of the model plant Arabidopsis thaliana coding for an ATP-binding cassette (ABC) transporter in which the ratio of sugars to secondary metabolites in the root exudates is altered compared to the wild-type (wt). We found that when Atpdr2 is grown in native Arabidopsis soils, a complete overhaul of the microbial community compared with wild-type Arabidopsis roots is seen. The Atpdr2 mutant promotes the growth of beneficial bacteria, such as plant growth and promoting rhizobacteria (PGPRs), nitrogen fixers and bacteria involved in heavy metal remediation. First, the project will address the influence of root-secreted chemical compounds and families from Atpdr2 and wt plants on the growth of beneficial soil microbes within the native microbial community. The second objective of the project is to characterize the Atpdr2 mutant to determine mechanisms used by the mutant to alter the ratio of sugars and secondary metabolites exuded by roots. These studies are transformative because they will provide mechanistic knowledge to develop new approaches by which crops can be genetically manipulated to culture soil microbes for beneficial purposes. Broader Impacts:This project will provide training for undergraduate and graduate students, and a post-doctoral trainee in research activities. These activities will be complemented with career development and mentoring workshops particularly targeted to graduate students and postdocs. In addition, the project will provide summer research and training activities for minority undergraduate students from the John Jay College of Criminal Justice of the City University of New York and educational activities for elementary students in Fort Collins. In the third year of the project, a colloquium will be conducted to discuss science, energy issues, third-world country involvement in energy topics, intellectual property, and international treaties related to microbial diversity.

智力优点：植物通过根部分泌物启动与土壤微生物群的相互作用。 因此，操纵植物根系分泌物提供了一个独特的机会，以最终有利于植物/作物健康的方式影响土壤微生物群落。该项目将通过表征编码 ATP 结合盒 (ABC) 转运蛋白的模式植物拟南芥突变体 (Atpdr2) 来提供此类工具，其中根分泌物中糖与次生代谢物的比例与野生型 (wt) 相比发生了改变。 我们发现，当 Atpdr2 在本地拟南芥土壤中生长时，与野生型拟南芥根相比，微生物群落发生了彻底的改变。 Atpdr2突变体促进有益细菌的生长，例如植物生长促进根际细菌（PGPR）、固氮剂和参与重金属修复的细菌。 首先，该项目将解决 Atpdr2 和 wt 植物根部分泌的化合物和家族对本地微生物群落内有益土壤微生物生长的影响。该项目的第二个目标是表征 Atpdr2 突变体，以确定突变体用于改变根部分泌的糖和次生代谢物比例的机制。这些研究具有变革性，因为它们将提供机械知识来开发新方法，通过这些新方法可以对作物进行基因操纵，以培养土壤微生物以达到有益的目的。更广泛的影响：该项目将为本科生和研究生以及博士后实习生提供研究活动培训。 这些活动将辅之以专门针对研究生和博士后的职业发展和指导研讨会。 此外，该项目还将为纽约市立大学约翰·杰伊刑事司法学院的少数族裔本科生提供暑期研究和培训活动，并为柯林斯堡的小学生提供教育活动。 在该项目的第三年，将举行一次研讨会，讨论科学、能源问题、第三世界国家参与能源主题、知识产权以及与微生物多样性相关的国际条约。