Integration of planar cell polarity and membrane trafficking during zebrafish gastrulation

斑马鱼原肠胚形成过程中平面细胞极性和膜运输的整合

• 批准号: 0950849
• 负责人: Jason Jessen
• 金额: $ 42.9万
• 依托单位: Vanderbilt University Medical Center
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0950849&HistoricalAwards=false
• 关键词: Integration; planar; cell; polarity; membrane

Animal development requires a diverse array of cell movement events to shape the body plan and generate an embryo with three distinct tissue layers. Significant among these movements is the process of collective migration whereby groups of polarized cells move in a directional manner while maintaining some level of contact with each other. However, the fundamental question of how this process is coordinated to promote the directional movement of large cell populations remains unanswered. This research project will address this question by utilizing the zebrafish as a genetic and embryological model system that can be manipulated using a variety of molecular techniques. In addition, the optical clarity of the zebrafish embryo affords the opportunity to perform microscopic imaging of cell migration in a live vertebrate animal. This project will determine (at both the cellular and biochemical levels) how specific proteins shown to be important for establishing cell polarity function to regulate collective or group cell migration events. This project is significant for the following reasons: (1) it will benefit society by providing novel insight into the complex process of collective cell migration, a process that is important for normal animal development and (2) it provides a unique training opportunity for post-doctoral scholars and undergraduate students to learn how to collect and analyze data from an animal model system.

动物的发育需要一系列不同的细胞运动事件来塑造身体计划，并产生具有三个不同组织层的胚胎。 在这些运动中，重要的是集体迁移的过程，由此极化细胞组以定向方式移动，同时保持彼此之间的某种程度的接触。 然而，如何协调这一过程以促进大细胞群的定向运动的根本问题仍然没有答案。 该研究项目将通过利用斑马鱼作为可以使用各种分子技术操纵的遗传和胚胎学模型系统来解决这个问题。 此外，斑马鱼胚胎的光学清晰度提供了在活脊椎动物中进行细胞迁移的显微成像的机会。 该项目将确定（在细胞和生物化学水平上）特定蛋白质如何对建立细胞极性功能起重要作用，以调节集体或群体细胞迁移事件。 该项目具有重要意义，原因如下：（1）它将通过对集体细胞迁移的复杂过程提供新的见解而造福社会，这一过程对正常动物发育至关重要;（2）它为博士后学者和本科生提供了一个独特的培训机会，学习如何收集和分析动物模型系统的数据。