Role of FHY3 and FAR1 in transcriptional regulation of phytochrome A signaling in Arabidopsis

项目摘要PI: Haiyang WangCo-PI: Matthew hudson提案号：os -0641639提案题目：FHY3和FAR1在拟南芥光敏色素A信号转录调控中的作用植物利用一系列光感受器感知周围光环境并进行适应性生长发育。光敏色素A （Phytochrome A, phyA）是高等植物中介导各种远红光诱导反应的主要光感受器。现在人们普遍认为，phyA的光激活启动了一个信号转导级联，导致受调节的基因表达变化和随后的光形态发育。FHY3和FAR1是两个同源蛋白，与MuDRA类转座酶具有显著的氨基酸相似性，它们定义了拟南芥中phyA信号传导的两个重要正调控因子。然而，它们的分子功能以及它们如何适应phyA信号通路仍然是难以捉摸的。该项目的中心目标是证实FHY3和FAR1可能定义了一类新的转录调控因子的假设，可能是从一个古老的Mutator-like转座元件驯化而来的，它们在细胞核中调节远红光下植物响应基因的表达和光形态形成的发育。三个具体目标是：1。研究FHY3和FAR1在植物细胞中的转录调控活性；2. 鉴定其直接靶基因并进行功能鉴定；和3。探讨它们与光感受器phyA的功能关系。这些研究结果将有助于更好地了解FHY3和FAR1在调控phyA介导的基因表达中的作用，并为建立连贯的phyA信号通路提供重要信息。这项工作预计会产生更广泛的影响，包括培养不同层次的科学家，从本科生到研究生和博士后。PI还将参与当地高中教师的培训，并在当地小学课堂上介绍由印第安纳大学教授罗杰·p·汉格特（Roger P. Hangarter）制作的“动态植物”电影，向学生讲授植物对光和其他环境信号的反应。

Project Abstract PI: Haiyang WangCo-PI: Matthew HudsonProposal Number: IOS-0641639Proposal Title: Role of FHY3 and FAR1 in transcriptional regulation of phytochrome A signaling in ArabidopsisPlants use an array of photoreceptors to sense the ambient light environment and undergo adaptive growth and development. Phytochrome A (phyA) is the primary photoreceptor for mediating various far-red light induced responses in higher plants. It is widely accepted now that photo-activation of phyA initiates a signal transduction cascade that leads to regulated gene expression change and subsequent photomorphogenic development. FHY3 and FAR1, two homologous proteins that share significant amino acid similarities with the MuDRA class of transposases, define two essential positive regulators of phyA signaling in Arabidopsis. However, their molecular function and how they fit in the phyA signaling pathway have remained elusive. The central goal of this project is substantiate the hypothesis that FHY3 and FAR1 may define a novel class of transcriptional regulators, possibly domesticated from an ancient Mutator-like transposable element, that act in the nucleus to regulate phyA-responsive gene expression and photomorphogenic development under far-red light. The three specific aims are: 1. To characterize the transcriptional regulatory activity of FHY3 and FAR1 in plant cells; 2. To identify their direct target genes and functional characterization; and 3. To investigate their functional relationship with the photoreceptor phyA. Results from these studies will lead to a better understanding of FHY3 and FAR1 in regulating phyA-mediated gene expression and add critical information toward establishing a coherent phyA signaling pathway. The broader impacts expected from this work include training of different levels of scientists, ranging from undergraduate students, to graduate students and postdoc fellows. The PI will also participate in the training of local high school teachers and introduce the "Plants-In-Motion" movies, which were created by Professor Roger P. Hangarter at Indiana University, in local elementary school classes to teach school children about plant responses to light and other environmental signals.