植物花干细胞负责产生特定数目的花器官后其干细胞活性被程序性终止，因此花干细胞是研究干细胞时序性调控的理想模型，同时对其调控机理的研究对作物高产也具有重要意义。在拟南芥的花分生组织中，WUSCHEL是调控花干细胞活性的关键基因；AGAMOUS和其它基因可通过时序性调控WUSCHEL的表达而抑制干细胞的活性，参与该过程的基因及分子机制还不很清楚。我们前期工作中利用一个AGAMOUS弱突变体ag-10，通过EMS诱变，筛选出一个花干细胞活性增强的突变体。图位克隆表明FHY3基因发生了终止突变，转基因恢复和等位基因验证表明该基因参与了花干细胞活性的调控；原位杂交证明该基因可调控WUS的表达。本研究将通过遗传学及高通量测序等多种分子生物学技术来研究FHY3在花干细胞维持和分化中的作用机制，最终通过FHY3靶基因的鉴定及功能分析揭示FHY3介导的花发育及干细胞分化的分子网络体系。

Floral stem cells are responsible for producing a defined number of floral organs, and then will be terminated programmatically at a particular developmental stage. Therefore, floral stem cells offer an ideal model to study the temporal control of stem cells maintenance, which is also very significant for the high-yielding of crops. In Arabidopsis floral meristem, WUSCHEL is a key gene for stem cells maintenance; AGAMOUS and other essential genes can repress stem cells maintenance by temporal control of WUSCHEL expression, however, the involved genes and molecular mechanism of this process is still not very clear. Based on an AG weak mutant ag-10 EMS mutagenesis, we screened a mutant with enhanced froral meristem indeterminacy of ag-10. Using map based cloning we detected a premature mutant occurring in FHY3 coding region; transgenic plant with over-expression of this gene and allele analysis show rescued or enhanced phenotype respectively, which suggested the mutation was responsible for mutant defect; in situ hybridization assay show the WUSCHEL expression was delayed due to the loss of function of FHY3.In this study, we will study the function of FHY3 and figure out the FHY3 target genes and their function in stem cells maintenance and determinacy by using a combinational approach of genetics , next generation sequencing and bioinformatics , our goal is to reveal the FHY3 mediated molecular net work in floral development, especially in floral meristem maintenance and determinacy.