Plasmodesmata and Cell Death: Role of PDLP5 as a Mediator

胞间连丝和细胞死亡：PDLP5 作为介质的作用

• 批准号: 0954931
• 负责人: Jung-Youn Lee
• 金额: $ 46.28万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-15 至 2016-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0954931&HistoricalAwards=false
• 关键词: Plasmodesmata; Cell; Death; Role; PDLP5

Plasmodesmata are plant-unique intercellular communication channels, which allow plant cells to share their cytoplasm and build a multicellular or supracellular organism. These fundamental structures are expected to play crucial roles in orchestrating physiological and developmental programs as well as in controlling systemic defense responses against pathogens. However, what molecular players mediate the signaling through this unique communication system and how this process is mechanistically controlled are not known. The focus of this project is on gaining new insight into how the regulation of plasmodesmal signaling is specifically linked to the onset or progression of programmed cell death induced during developmental progression or by defense responses. By employing novel molecular tools, this project will attempt to correlate the inhibition of plasmodesmal function with controlled cell deaths. The two pressing questions driving this project are: what is the role of plasmodesmal signaling in cell death during defense response? How does a signal sensed at or transduced to the plasmodesmata link to a cell death pathway? Successful outcomes of this research project would provide compelling insights into the role of intercellular communication in death and survival of plant cells. In addition, new discoveries resulting from this project will also contribute to gaining a fundamental insight into how different organisms have independently evolved to meet the same goal of survival as multicellular organisms. As plasmodesmal function is essential for both cell-to-cell communication and long-distance signaling in plants, insights gained by this study could lead to the development of new agricultural technology. For example, manipulation of plants for resistance to the spread of pathogens could be achieved. This research project will actively engage undergraduate and graduate students in cutting-edge research and scientific communications as well as K-12 students in firsthand cell biology experience.

胞间连丝是植物特有的细胞间通讯通道，它使植物细胞能够共享细胞质，形成多细胞或超细胞生物体。这些基本结构预计将在协调生理和发育程序以及控制对病原体的系统防御反应中发挥关键作用。然而，哪些分子参与者通过这个独特的通讯系统介导信号传递，以及这个过程是如何机械控制的尚不清楚。该项目的重点是获得新的见解，了解胞间连丝信号的调节如何与发育过程中诱导的程序性细胞死亡的发生或进展或防御反应有关。通过使用新的分子工具，该项目将试图将胞间连丝功能的抑制与受控的细胞死亡相关联。推动这个项目的两个紧迫问题是：在防御反应中，胞间连丝信号在细胞死亡中的作用是什么？在胞间连丝上感受到的或传递到胞间连丝的信号是如何与细胞死亡途径联系起来的？该研究项目的成功结果将为细胞间通讯在植物细胞死亡和存活中的作用提供令人信服的见解。此外，该项目产生的新发现也将有助于从根本上了解不同的生物体如何独立进化，以满足与多细胞生物相同的生存目标。由于胞间连丝的功能对于植物中的细胞间通讯和远距离信号传导都是必不可少的，因此这项研究所获得的见解可能会导致新农业技术的发展。例如，可以操纵植物以抵抗病原体的传播。该研究项目将积极吸引本科生和研究生参与前沿研究和科学交流，以及K-12学生参与第一手细胞生物学经验。