Coenzyme B12-Dependent 1,2-Propanediol Degradation by Salmonella

沙门氏菌对辅酶 B12 依赖性 1,2-丙二醇的降解

• 批准号: 0956451
• 负责人: Thomas Bobik
• 金额: $ 68万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-15 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0956451&HistoricalAwards=false
• 关键词: Coenzyme; B12; Dependent; Propanediol; Degradation

Intellectual Merit:Bacterial microcompartments are simple organelles used to optimize metabolic processes by sequestering toxic or volatile pathway intermediates. They consist of a protein shell that encases metabolic enzymes. The research will focus on understanding the structural and assembly principles of bacterial microcompartments as well as the mechanism by which the encapsulated enzymes are supplied with needed cofactors. The production of toxic pathway intermediates is a problem in the production of certain green chemicals by bacteria. Microcompartments are a natural system that solves this problem. Hence, understanding their molecular principles could lead to the genetic engineering of designer microcompartments for the improved production of green chemicals. A second focus of the research is to elucidate the biochemical properties of proteins required to activate vitamin B12 to coenzyme B12. This process is required for the activity of coenzyme B12-dependent enzymes which are widespread in nature and play important roles in the environment and in biotechnology. Thus, an improved understanding of vitamin B12 activation could result in better methods for the production of green chemicals and for bioremediation of contaminated habitats.Broader impacts:Two graduate students, one post-doctoral fellow, and two undergraduates will carry out the research under guidance for the principle investigator. This will provide students with extensive training in the area of bacterial metabolism and genetics. This field is expected to be increasingly important as the need to engineer microbes for the production of chemicals and biofuels from renewable resources grows in the coming years. The PI will also work with the Iowa State University Carver Foundation to provide summer research opportunities to two minority undergraduate students per year.

智力优势：细菌微室是一种简单的细胞器，用于隔离有毒或挥发性途径的中间产物，以优化代谢过程。它们由包裹新陈代谢酶的蛋白质外壳组成。这项研究将侧重于了解细菌微室的结构和组装原理，以及微囊化的酶被提供所需辅因子的机制。在细菌生产某些绿色化学品的过程中，有毒途径中间体的产生是一个问题。微型隔间是解决这一问题的自然系统。因此，了解它们的分子原理可能会导致设计微隔室的基因工程，以改进绿色化学品的生产。这项研究的第二个重点是阐明将维生素B12激活为辅酶B12所需蛋白质的生化特性。这一过程是辅酶B12依赖的酶活性所必需的，这些酶在自然界中广泛存在，并在环境和生物技术中发挥重要作用。因此，更好地了解维生素B12的激活可以产生更好的方法来生产绿色化学品和对受污染的栖息地进行生物修复。广泛的影响：两名研究生、一名博士后研究员和两名本科生将在首席研究员的指导下进行研究。这将为学生在细菌新陈代谢和遗传学领域提供广泛的培训。随着未来几年对利用可再生资源生产化学品和生物燃料的微生物的需求增加，这一领域预计将变得越来越重要。PI还将与爱荷华州立大学卡弗基金会合作，每年为两名少数族裔本科生提供暑期研究机会。