MRI-R2: Acquisition of a Confocal/Multiphoton Microscope to Advance Cellular and Physiological Research at Marshall University

MRI-R2：马歇尔大学购买共焦/多光子显微镜以推进细胞和生理学研究

• 批准号: 0959012
• 负责人: Michael Norton
• 金额: $ 93.01万
• 依托单位: Marshall University Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-15 至 2012-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959012&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; Confocal; Multiphoton

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Support from the NSF MRI-R2 program has allowed Marshall University to purchase a Leica TCS SP5 II AOBS and MP High End System. This system includes the ability to perform very high resolution imaging, and very fast imaging for the rapid detection, with good temporal resolution, of fast physiological changes that can last 1 msec or less. These features thus allow the combination of fluorescent imaging in live tissues with other physiological techniques required by Marshall researchers. The initial user group for the new system consists of 5 major and 10 minor users, representing researchers from 5 departments, with projects that span a broad range of biological and biochemical research in neurobiology, genetics, physiology, molecular biology, and bioengineering. Major projects use multiphoton microscopy to study signal transduction mechanisms in single neurons, and to analyze the effect of environmental variables on bone elongation by imaging skeletal growth plates in vivo. Other research projects that benefit from the high resolution imaging, multiple color channels, rapid scanning, and precise optical sectioning capability of the proposed instrumentation include a study using the fruit fly Drosophila as a model to investigate the orientation and shape of cells within the plane of an epithelium; an analysis of stem cell integration and differentiation in tissues; and the evaluation of DNA damage repair mechanisms. Additional projects include the intracellular localization of an important cell cycle regulatory protein in the protist Acanthamoeba; molecular signaling triggered by oxidized dietary lipids; neuronal mechanisms underlying the regulation of body load in stick insects; and the development of single-molecule sensor systems. The new equipment enhances the cutting edge research programs and student research opportunities. The projects include 17 undergraduate and 23 graduate student researchers. Confocal microscopy is regularly used in coursework (laboratory exercises, and capstone and independent research projects) at Marshall, and as part of outreach programs. These programs include the Marshall Chapter of the WV Space Grant Consortium's NASA Research Scholarships Program; the NSF Kentucky-WV Louis Stokes Alliance for Minority Participation; the WV-IDeA Network of Biomedical Research Excellence; The Biomedical Sciences Summer Research Internship for Minority Students; and the state-funded Marshall Summer Undergraduate Research Experience. In addition, summer Bridge programs expose high school students, their teachers, and Marshall's rising sophomores to cutting-edge research which includes the use of confocal microscopy. Publication and presentation are major dissemination criteria, and all participating faculty have support student presenters at national and/or international conferences as well as at local events such as Undergraduate Research Day at the WV Capitol.

该奖项由2009年美国复苏和再投资法案（公法111-5）资助。NSF MRI-R2计划的支持使马歇尔大学能够购买Leica TCS SP 5 II AOBS和MP高端系统。该系统包括执行非常高分辨率成像的能力，以及用于快速检测的非常快的成像，具有良好的时间分辨率，可以持续1毫秒或更短的快速生理变化。这些特征因此允许将活组织中的荧光成像与马歇尔研究人员所需的其他生理技术相结合。新系统的初始用户组由5个主要用户和10个次要用户组成，代表来自5个部门的研究人员，其项目涵盖神经生物学，遗传学，生理学，分子生物学和生物工程等广泛的生物和生物化学研究。主要项目使用多光子显微镜研究单个神经元的信号转导机制，并通过体内骨骼生长板成像分析环境变量对骨伸长的影响。其他研究项目受益于高分辨率成像，多个颜色通道，快速扫描，和精确的光学切片能力的建议仪器包括一项研究使用果蝇作为模型，以调查上皮细胞的平面内的细胞的方向和形状;组织中的干细胞整合和分化的分析;和DNA损伤修复机制的评估。其他项目包括一个重要的细胞周期调节蛋白质在原生生物阿米巴的细胞内定位;氧化的饮食脂质引发的分子信号传导;在竹节虫的身体负荷调节的神经元机制;和单分子传感器系统的发展。新设备增强了尖端的研究计划和学生的研究机会。这些项目包括17名本科生和23名研究生研究人员。共焦显微镜经常用于课程（实验室练习，顶点和独立的研究项目）在马歇尔，并作为推广计划的一部分。这些计划包括西弗吉尼亚州空间赠款财团的美国宇航局研究奖学金计划的马歇尔章; NSF肯塔基州-西弗吉尼亚州路易斯斯托克斯少数民族参与联盟; WV-IDEA生物医学研究卓越网络;少数民族学生的生物医学科学暑期研究实习;和国家资助的马歇尔暑期本科生研究经验。此外，夏桥计划暴露高中学生，他们的老师，和马歇尔的上升的追随者，以尖端的研究，其中包括使用共聚焦显微镜。出版物和演示文稿是主要的传播标准，所有参与的教师都在国家和/或国际会议以及当地活动中支持学生演讲者，如WV国会大厦的本科生研究日。