MRI-R2: Acquisition of an epifluorescence microscope for research and training at Gettysburg College

MRI-R2：购买落射荧光显微镜用于葛底斯堡学院的研究和培训

• 批准号: 0959175
• 负责人: J. Matthew Kittelberger
• 金额: $ 15.98万
• 依托单位: Gettysburg College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-15 至 2012-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959175&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; epifluorescence; microscope

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Gettysburg College has received support from the NSF MRI-R2 program to support the acquisition of a Nikon Eclipse 90i microscope plus digital imaging and software technology. This microscope will enable new research initiatives for six faculty, and broaden and enhance the undergraduate training environment at Gettysburg College. The Eclipse 90i microscope will allow our faculty and their undergraduate collaborators to immediately pursue a variety of new research initiatives that are currently not possible. Dr. Kittelberger and his students study the structure and function of neural circuits involved in vocal communication in teleost fish, focusing on neurophysiological mechanisms by which neuromodulators shape vocal behavior. Dr. Frey and her students currently use lipid monolayers to study the physical and chemical interactions between molecules that lead to the formation of cell membrane lipid rafts, ordered microdomains thought to play a role in membrane transport and signal transduction. Dr. James and his students will pursue recent insights into cell cycle control, DNA damage responses, and meiotic recombination, using the model eukaryotic fungus Aspergillus nidulans, as follows: (1) a novel fungal DNA ligase that plays an essential role in meiosis; (2) novel regulatory mechanisms by which Rif1, a component of an S-phase DNA damage response, appears to inhibit the activity of Dbf4-dependent kinase (DDK) to trigger DNA synthesis and meiotic recombination; and (3) in collaboration with Dr. SL McGuire (Millsaps College), an unsuspected interaction between the canonical cell cycle regulator, cdc2-cyclinB kinase, and a component of the mRNA export machinery, Hrb1. Dr. Siviy and his students study how social variables modulate fear and anxiety in young rats. In conjunction with studies on behavioral indices of fear and anxiety, acquisition of this microscope will allow them to quantify changes in neural activation following exposure to an innate fear stimulus in the brain of young rats and to determine how early social experiences can modulate this activation. Dr. Brandauer studies the potential of skeletal muscle to release signaling proteins called myokines and Dr. Powell studies signaling pathways of the innate immune response in C. elegans. Gettysburg College is liberal arts institution offering 4-year bachelor degree programs, with a total enrollment of ~2500 undergraduates. Through coursework and independent research, the biology, chemistry, biochemistry and molecular biology (BMB), and neuroscience curricula prepare students for continued work in these disciplines. Through close faculty-student collaboration and by offering outstanding research opportunities, Gettysburg College effectively prepares our science students to become leaders among the next generation of scientists; and (2) attracts greater numbers of undergraduates, including women and underrepresented minorities, to the sciences. Acquisition of the Eclipse 90i microscope contributes vitally to these objectives by exposing students to new areas of scientific inquiry and by providing sophisticated, discovery-based training with the latest technology and instrumentation. Results from the studies enabled by the new microscope will be published in peer-reviewed journals and presented by students and faculty at regional and national meetings.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。葛底斯堡学院已获得NSF MRI-R2计划的支持，以支持尼康Eclipse 90 i显微镜加上数字成像和软件技术的收购。这台显微镜将为六名教师提供新的研究计划，并扩大和加强葛底斯堡学院的本科生培训环境。Eclipse 90 i显微镜将允许我们的教师和他们的本科合作者立即追求各种新的研究计划，目前是不可能的。 Kittelberger博士和他的学生研究硬骨鱼中参与声音交流的神经回路的结构和功能，重点是神经调节剂塑造声音行为的神经生理学机制。 Frey博士和她的学生目前使用脂质单层来研究导致细胞膜脂筏形成的分子之间的物理和化学相互作用，这些脂筏被认为在膜运输和信号转导中发挥作用。 James博士和他的学生将利用真核真菌构巢曲霉（Aspergillusnidulans）来研究细胞周期控制、DNA损伤反应和减数分裂重组的最新见解，具体如下：（1）一种在减数分裂中起重要作用的新型真菌DNA连接酶;（2）新的调节机制，通过该机制，S期DNA损伤反应的组分Rif 1，似乎抑制Dbf 4依赖性激酶（DDK）触发DNA合成和减数分裂重组的活性;以及（3）与SL McGuire博士合作（Millsaps College），一种典型的细胞周期调节因子cdc 2-cyclinB激酶和mRNA输出机制的组分之间的意外相互作用，Hrb1。 Siviy博士和他的学生研究了社会变量如何调节年轻大鼠的恐惧和焦虑。结合对恐惧和焦虑行为指数的研究，获得这种显微镜将使他们能够量化暴露于幼年大鼠大脑中先天恐惧刺激后神经激活的变化，并确定早期社会经验如何调节这种激活。 Brandauer博士研究骨骼肌释放称为肌因子的信号蛋白的潜力，Powell博士研究C.优雅的 葛底斯堡学院是一所文科院校，提供4年制学士学位课程，本科生总入学人数约为2500人。通过课程和独立研究，生物学，化学，生物化学和分子生物学（BMB），神经科学课程为学生在这些学科的继续工作做好准备。通过密切的师生合作，并通过提供优秀的研究机会，葛底斯堡学院有效地准备我们的科学学生成为下一代科学家中的领导者;（2）吸引更多的本科生，包括妇女和代表性不足的少数民族，科学。Eclipse 90 i显微镜的收购通过让学生接触科学探究的新领域，并通过提供最新技术和仪器的复杂，基于发现的培训，为这些目标做出了重要贡献。 由新显微镜实现的研究结果将发表在同行评议的期刊上，并由学生和教师在区域和国家会议上介绍。