MRI-R2: Acquisition of High-throughput Crystallization Instrumentation to Enhance the Macromolecular X-ray Crystallography Facility of the University of Puerto Rico

MRI-R2：购置高通量结晶仪器以增强波多黎各大学的大分子 X 射线晶体学设施

• 批准号: 0959254
• 负责人: Abel Baerga-Ortiz
• 金额: $ 51.67万
• 依托单位: University of Puerto Rico-Rio Piedras
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959254&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; throughput; Crystallization

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the acquisition of high-throughput crystallization instrumentation to enhance the Macromolecular X-ray Crystallography Facility (MXCF) of the University of Puerto Rico (UPR). The long-term goal of this facility is to bring a productive structural biology research component to the University of Puerto Rico, which will impact a wide range of research efforts. Structural biology researchers at the facility are investigating a wide scope of projects, including non-genetic mechanisms of protein sequence variability, enzyme function, and related topics. The facility plays a central role in graduate education, training workshops in x-ray crystallography and structural biology, and training of faculty, postdoctoral fellows, graduate students and undergraduates. This instrumentation promotes collaboration of investigators from four research centers in San Juan, Puerto Rico, and prestigious research institutes in the US with the common theme of solving important problems through structure determination using x-ray crystallography. The faculty involved in this project are actively engaged in training efforts with a large population of underrepresented minority students at the University of Puerto Rico. The MXCF will continue to promote the expansion of high-quality, collaborative structural biology research among the University of Puerto Rico scientific community, strengthening multidisciplinary collaborations among investigators within UPR-Río Piedras and other institutions inside and outside Puerto Rico. Undergraduates, graduate students, postdoctoral fellows, and faculty will be trained in modern methods of macromolecular crystallization and structure determination via a new course and a workshop series that will include hands-on experience with the newly acquired instrumentation. Results from these studies will be disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.

该奖项是根据2009年《美国复苏与再投资法》（公法111-5）资助的。这项主要的研究仪器恢复和再投资（MRI-R2）授予了高通量结晶仪器的获取，以增强波多黎各大学（UPR）的大分子X射线晶体学设施（MXCF）。该设施的长期目标是为波多黎各大学带来一个富有成效的结构生物学研究部分，这将影响广泛的研究工作。该设施的结构生物学研究人员正在研究广泛的项目范围，包括蛋白质序列变异性，酶功能和相关主题的非遗传机制。该设施在研究生教育，X射线晶体学和结构生物学的培训讲习班以及教师，博士后研究员，研究生和本科生中发挥着核心作用。该仪器促进了来自圣胡安，波多黎各的四个研究中心的调查人员的合作，以及美国享有声望的研究机构的合作，其共同主题是通过使用X射线晶体学来确定结构，以解决重要问题。参与该项目的教职员工正在积极从事培训工作，波多黎各大学的大量人数不足的少数族裔学生。 MXCF将继续促进波多黎各大学科学界之间高质量的，协作的结构生物学研究的扩展，从而加强了Upr-RíoPiedras与波多黎各内外的其他机构之间的研究人员之间的多学科合作。本科生，研究生，博士后研究员和教职员工将通过新课程和研讨会系列进行现代大分子结晶和结构确定的培训，其中将包括新获得的仪器的动手经验。这些研究的结果将由学生和教职员工在区域和国家会议上以及通过同行评审期刊的出版中传播。