MRI-R2: Acquisition of a High Performance Liquid Chromatography/Tandem Mass Spectrometry System

MRI-R2：购置高性能液相色谱/串联质谱系统

• 批准号: 0959401
• 负责人: Deon Miles
• 金额: $ 35万
• 依托单位: University of the South
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959401&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; Performance; Liquid

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research Instrumentation (MRI) program Professor Deon Miles and colleague Robert E. Bachman from the University of the South will acquire a high performance, liquid-chromatograph, tandem mass spectrometer (HPLC/MS/MS) capable of handling high molecular mass materials. The instrument will be used to support research activities such as: 1) solution aggregates formed by platinum diimine complexes, 2) separation and identification of water-soluble semiconducting and metallic nanoparticles, 3) isolation and characterization of cardiolipin from skeletal muscle membrane phospholipid fatty acids, and 4) identification of S-nitrosylated proteins and their degree of nitrosylation.Mass spectrometry (MS) is used to identify the chemical composition of a sample and determine its purity by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. High pressure liquid chromatography is an isolation technique that precedes the mass spectrometry analysis. It separates a mixture into its several constituent chemicals which are then analyzed by the mass spectrometer. These are widely used analytical techniques to identify and quantify the chemical composition of a sample. It is important for students to be trained using modern instrumentation. The research will have a significant impact in a number of areas, especially chemistry, materials chemistry and biochemistry. This instrument will be an integral part of teaching as well as research.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）。来自南方大学的Bachman将获得一台能够处理高分子量材料的高效液相色谱串联质谱仪（HPLC/MS/MS）。该工具将用于支持研究活动，例如：1）由铂二亚胺络合物形成的溶液聚集体，2）水溶性半导体和金属纳米颗粒的分离和鉴定，3）从骨骼肌膜磷脂脂肪酸中分离和表征心磷脂，和4）S-亚硝基化蛋白质的鉴定及其亚硝基化程度。质谱（MS）用于鉴定样品的化学成分，并通过测量样品中分子成分的质量来确定其纯度，这些分子成分被电离并由质谱仪检测。高压液相色谱法是一种先于质谱分析的分离技术。它将混合物分离成几种化学成分，然后由质谱仪进行分析。这些是广泛使用的分析技术，用于识别和量化样品的化学成分。对学生进行使用现代仪器的训练是很重要的。该研究将在许多领域产生重大影响，特别是化学，材料化学和生物化学。该仪器将成为教学和研究的一个组成部分。