MRI-R2: Acquisition of a liquid chromatograph-mass spectrometer for biogeochemistry and paleoclimate studies at the Large Lakes Observatory

MRI-R2：在大湖天文台购买用于生物地球化学和古气候研究的液相色谱-质谱仪

• 批准号: 0959984
• 负责人: Josef Werne
• 金额: $ 32.73万
• 依托单位: University of Minnesota Duluth
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2012-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959984&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; liquid; chromatograph

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."The PI?s request funding to acquire an up-to-date liquid chromatograph ? mass spectrometer (LC/MS). Specifically, we request a triple quadrupole LC/MS system with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) sources, thus allowing characterization of a wide range of soluble molecules, including extracted lipids that are not volatile enough to be GC-amenable. The acquisition of this instrument will allow characterization of molecules of widely varying structures, while keeping maintenance requirements at a level supportable with our current infrastructure and technical support staff. Ongoing and future projects include analysis of lipids for development and application of paleoenvironmental proxies, studies of water column particulate and dissolved organic matter, studies of intact polar lipids, and further development of black carbon measurements.Broader Impacts: The acquisition of the LC/MS triple quad system will open up new avenues of research into compounds that are not amenable for GC/MS analyses. The significance of the research could be far-reaching with respect to environmental concerns over black carbon. Teaching undergraduate and graduate research is a real strength of both PI's. The PI's demonstrated priority emphasis on hands-on experience, including research experiences for graduates and undergraduates in three different courses. The attitude that instrumentation should not be used as a 'black box' but rather as a hands-on learning opportunity pervades the text, and this is an important plus for this proposal. This aspect meets one of the principal NSF strategies for fostering the integration of research and education.

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。”PI？我们要求拨款购买一台最新的液相色谱仪？质谱仪（LC/MS）。具体而言，我们要求一个三重四极杆LC/MS系统与电喷雾电离（ESI），大气压化学电离（APCI）源，从而允许表征范围广泛的可溶性分子，包括提取的脂质是不够挥发，是GC-顺从。该仪器的获得将允许表征各种结构的分子，同时将维护要求保持在我们现有基础设施和技术支持人员可支持的水平。正在进行和未来的项目包括为开发和应用古环境代用指标而进行的脂类分析、水柱颗粒和溶解有机物的研究、完整极性脂类的研究以及进一步开发黑碳测量。 LC/MS三重四系统的收购将开辟新的途径，研究化合物，不适合GC/MS分析。这项研究的意义可能是深远的方面对黑碳的环境问题。教授本科生和研究生的研究是两个PI的真实的优势。PI表现出优先重视实践经验，包括研究生和本科生在三个不同课程的研究经验。仪表不应该被用作“黑匣子”，而是作为一个动手学习的机会贯穿文本的态度，这是这个建议的一个重要优势。这方面符合NSF促进研究和教育一体化的主要战略之一。