MRI-R2: Acquisition of equipment for investigating biodynamic interactions

MRI-R2：购买用于研究生物动力学相互作用的设备

• 批准号: 0960089
• 负责人: Carole Cramer
• 金额: $ 107.09万
• 依托单位: Arkansas State University Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0960089&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; equipment; investigating

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Support from the NSF MRI-R2 program has allowed the purchase of a Beckman Coulter MoFlo XDP cell sorter, a BD Pathway 885 High-Content Bioimager, and a Miltenyi Gentle Macs Dissociator, and related equipment to support research focused on understanding the biodynamics of molecular interactions leading to cellular differentiation. This interdisciplinary effort integrates faculty, post-doctoral and student researchers in the disciplines of biochemistry, immunology, physiology, molecular cell biology, neurobiology and plant biology. Results of studies planned for this suite of instruments will allow specific subsets of T cells to be identified, separated, cultured, and their interactions with specific activating and inhibiting factors imaged in real time. The differential effects of growth factors on freshly isolated subpopulations of dorsal root ganglion neurons grown on carbon and magnetic nanotube substrates will be characterized. The uptake of plant lectins and fluorescent nanocrystals by pulmonary and immune cells in intact animals and the subsequent effects of this absorption on cellular molecular dynamics will be characterized. The equipment will also be used to support science education outreach to high school, undergraduate and graduate students. ASU has active NSF-funded REU, URM, GK-12, and Louis Stokes Minority Alliance programs, as well as a recently funded McNair project, that focus on recruiting, retaining and enhancing the educational experience of students of under-represented minorities. Many of these students are actively participating in research projects in the PIs labs. To further broaden potential impacts, training and applications seminars will be held in addition to integrating these technologies into our Molecular Biosciences PhD techniques courses in immunology and microscopy. Enhanced research capabilities will strengthen the University's effort to recruit and retain minority students, faculty, and collaborators who might otherwise choose other universities or other disciplines to study. Results from the studies will be published in peer-reviewed journals and presented by students and faculty at regional and national meetings.

该奖项是根据2009年美国复苏和再投资法案资助的（公法111-5）。NSF MRI-R2计划的支持允许购买Beckman Coulter MoFlo XDP细胞分选仪、BD Pathway 885高含量生物成像仪和Miltenyi Gentle Macs解离器，和相关设备，以支持研究重点是了解导致细胞分化的分子相互作用的生物动力学。这种跨学科的努力整合了生物化学，免疫学，生理学，分子细胞生物学，神经生物学和植物生物学学科的教师，博士后和学生研究人员。 这套仪器计划的研究结果将允许识别、分离、培养特定的T细胞亚群，并将它们与特定的激活和抑制因子的相互作用真实的成像。将表征生长因子对在碳和磁性纳米管基底上生长的背根神经节神经元的新鲜分离的亚群的差异影响。植物凝集素和荧光纳米晶体的吸收肺和免疫细胞在完整的动物和随后的影响，这种吸收对细胞分子动力学的特点。 这些设备还将用于支持对高中、本科和研究生的科学教育推广。亚利桑那州立大学有积极的NSF资助的REU，URM，GK-12和路易斯斯托克斯少数民族联盟计划，以及最近资助的麦克奈尔项目，重点是招聘，保留和提高代表性不足的少数民族学生的教育经验。这些学生中的许多人积极参与PI实验室的研究项目。为了进一步扩大潜在的影响，除了将这些技术整合到我们的分子生物科学博士技术课程免疫学和显微镜外，还将举办培训和应用研讨会。增强的研究能力将加强大学的努力，以招募和留住少数民族学生，教师和合作者，否则他们可能会选择其他大学或其他学科学习。 研究结果将发表在同行评审的期刊上，并由学生和教师在区域和国家会议上提出。