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DISSERTATION RESEARCH: RAD Phylogenetics: Harnessing Next-Generation Sequencing for Molecular Systematics

论文研究：RAD 系统发育学：利用下一代测序进行分子系统学

基本信息

批准号：
1011517
负责人：
Michael Sorenson
金额：
$ 1.32万
依托单位：
Trustees of Boston University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2013-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1011517&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH RAD Phylogenetics Harnessing

项目摘要

DNA sequences are now routinely used to reconstruct the branching evolutionary relationships among species, often displayed graphically as a phylogenetic tree. Phylogenetic trees are often based on just one or a few genetic loci, each representing a very small portion of the full genetic complement of a species. These small samples may produce misleading results due to complications arising from processes such as random genetic drift, natural selection, and hybridization. For some time, it has been recognized that the ideal solution would be to construct phylogenies using a large number of genetic loci sampled throughout the genome, but the cost of this approach has been prohibitive. Using two groups of bird species with different modes of reproduction and rates of speciation, this research will test the efficacy of a new "next-generation" sequencing method as a low-cost solution for recovering and sequencing essentially the same set of thousands of genetic loci from related species.Phylogenetic trees are critical to understanding the phenomenal diversity and evolutionary history of life on earth. The potentially powerful new tool evaluated by this research is likely to be of broad interest as it should be applicable with little or no modification to almost any organism and will produce data sets orders of magnitude larger than in the best current studies.

DNA序列现在通常用于重建物种之间的分支进化关系，通常以系统发育树的形式显示。系统发生树通常基于一个或几个遗传位点，每个基因位点代表一个物种完整遗传互补的一小部分。由于随机遗传漂变、自然选择和杂交等过程的复杂性，这些小样本可能会产生误导性的结果。一段时间以来，人们已经认识到，理想的解决方案是使用在整个基因组中采样的大量遗传基因座来构建同源性，但是这种方法的成本过高。利用两组具有不同繁殖模式和物种形成速率的鸟类物种，这项研究将测试一种新的“下一代”测序方法的有效性，这种方法是一种低成本的解决方案，可以从相关物种中恢复和测序基本相同的数千个基因位点。系统发生树对于理解地球上生命的惊人多样性和进化历史至关重要。这项研究评估的潜在强大的新工具可能会引起广泛的兴趣，因为它应该适用于几乎任何生物体，很少或没有修改，并将产生比目前最好的研究大几个数量级的数据集。