Capillary-Channeled Polymer (C-CP) Fiber Stationary Phases for High Speed and Preparative Protein Separation

克莱姆森大学的Ken Marcus教授及其合作者得到了化学测量和成像计划(化学系)和化学和生物分离计划(化学、生物工程、环境和运输系统系)的支持，并得到了实验计划的共同资助，以刺激竞争研究，开发毛细通道聚合物(C-CP)纤维并对其进行表征，作为快速处理生物大分子的固定相。C-CP材料适用于多种柱型(从微孔到制备)，并为大分子分离(相对于传统的多孔珠、膜和整体固定相)提供了优势，包括低流动阻力、消除尺寸排斥效应、高得多的传质速率以及高流动相速度(不一定是体积流速)和梯度速率而不牺牲色谱性能。这些特性使C-CP纤维柱作为二维高效液相色谱分离蛋白质的第二维具有潜在的应用价值。表面化学成分可以变化，以影响分离，同时提供高水平的灵活性。最后，对于高速和制备性分离(例如蛋白质的分离)等应用来说，该方法应该被证明是相对便宜的。这些研究涉及化学系和材料科学与工程学院的研究小组之间的合作，这两个学院都隶属于克莱姆森大学纤维和薄膜高级工程中心(CAEFF)。这项工作的成功完成可能会在南卡罗来纳州和其他地方的纺织和制药行业带来新的商业冒险。CAEFF项目让研究生在科学/工程界面上接触到科学和研究哲学。构建C-CP纤维柱的低成本和简单性将使它们能够用于新设计的本科生和研究生实验室实验，说明蛋白质层析的基本原理。

Prof. Ken Marcus and collaborators at Clemson University are supported by the Chemical Measurement and Imaging Program (Division of Chemistry) and the Chemical and Biological Separations Program (Division of Chemical, Bioengineering, Environmental, and Transport Systems), with co-funding from the Experimental Program to Stimulate Competitive Research, to develop and characterize capillary-channeled polymer (C-CP) fibers as stationary phases for rapid processing of biomacromolecules. C-CP materials are amenable to a wide range of column formats (microbore to preparative) and offer advantages for macromolecular separations (relative to traditional porous-bead, membrane, and monolithic stationary phases), including low flow resistance, elimination of size-exclusion effects, much higher mass transfer rates, and high mobile phase velocities (not necessarily volume flow rates) and gradient rates without sacrificing chromatographic performance. These properties make C-CP fiber columns potentially useful as the second dimension for 2-D HPLC protein separations. Surface chemistries can be varied to affect separation while providing high levels of flexibility. Finally, the approach should prove relatively inexpensive for applications such as high speed and preparative separations (e.g., of proteins). The studies involve a collaborative effort between research groups from the Department of Chemistry and the School of Materials Science and Engineering, both of which are affiliated with the Clemson University Center for Advanced Engineering of Fibers and Films (CAEFF). Successful completion of the work may lead to new commercial ventures in the textile and pharmaceutical industries in South Carolina and elsewhere. CAEFF programs expose graduate students to science and research philosophies across the science/engineering interface. The low cost and simplicity of constructing C-CP fiber columns will allow their use in newly designed undergraduate and graduate laboratory experiments illustrating the basic principles of protein chromatography.