SBIR Phase I: Engineering Thermophilic Geobacillus to Secrete an Enzyme Cocktail for Industrial Biomass Conversions

SBIR 第一阶段：改造嗜热土芽孢杆菌以分泌用于工业生物质转化的酶混合物

• 批准号: 1013185
• 负责人: Phillip Brumm
• 金额: --
• 依托单位: C5-6 TECHNOLOGIES
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1013185&HistoricalAwards=false
• 关键词: SBIR; Phase; Engineering; Thermophilic; Geobacillus

This SBIR Phase I project will genetically engineer a thermophilic bacterial strain capable of expressing and secreting all the enzymes necessary for biomass hydrolysis and that can grow on minimal media in aerobic and anaerobic conditions. The National Energy Plan Renewable Fuel Standard mandates the production of 21 billion gallons of advanced biofuels from non-food based crops by 2022. In order to achieve this goal, an economically feasible process to hydrolyze biomass must be developed. Production of cellulosic ethanol currently involves pretreating biomass with steam, acid or base to make it more accessible to enzymatic digestion. Enzymes, namely those possessing cellulase, xylanase, â-xylosidase and â-glucosidase activities, will be added after pretreatment to hydrolyze the biomass to its constituent five and six carbon sugars that can be fermented to produce fuel.The broader/commercial impact of the project will be low cost bio-fuel made from local biomass. This could reduce and stabilize energy prices, strengthen the national energy security, reduce the fiscal deficit, create American jobs and promote economic development.

这个SBIR第一阶段项目将对一种嗜热细菌菌株进行基因工程，该菌株能够表达和分泌生物质水解所需的所有酶，并能在好氧和厌氧条件下在最低限度的培养基上生长。国家能源计划可再生燃料标准要求到2022年从非食品类作物中生产210亿加仑的先进生物燃料。为了实现这一目标，必须开发一种经济上可行的生物质水解法。目前，纤维素乙醇的生产包括用蒸汽、酸或碱对生物质进行预处理，使其更容易被酶消化。具有纤维素酶、木聚糖酶、木糖苷酶和葡萄糖苷酶活性的酶将在预处理后加入，以将生物质水解为其组成的五和六碳糖，可发酵生产燃料。该项目的更广泛/更广泛的商业影响将是由当地生物质制成的低成本生物燃料。这可以降低和稳定能源价格，加强国家能源安全，减少财政赤字，创造美国就业机会，促进经济发展。