Simkania negevensis containing vacuoles: Formation, trafficking and subversion of host signaling

Simkania negevensis 含有液泡：宿主信号传导的形成、运输和颠覆

• 批准号: 198256059
• 负责人: Professor Dr. Thomas Rudel
• 金额: --
• 依托单位: Lehrstuhl für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/198256059?language=en
• 关键词: Simkania; negevensis; containing; vacuoles; Formation

It is still largely unknown how obligate intracellular bacteria of the order Chlamydiales establish and maintain their replicative vacuole in host cells. Simkania negevensis belongs to this group of bacteria and is closely related to the pathogenic Chlamydophila pneumoniae and Chlamydia trachomatis. Simkania infects both mammalian cells and amoeba, and thus represents a barely characterized evolutionary intermediate between the pathogenic and environmental members of the Chlamydiales. Our recent data demonstrated that Simkania replicates in an unusual vacuole which is located within the endoplasmic reticulum and is tightly connected to the host mitochondria. Here, we investigate the molecular mechanisms underlying the accommodation of the Simkania-containing vacuole (SCV). We will explore the formation and trafficking of the SCV and the interaction with the host endoplasmic reticulum and mitochondria. Further, the basis of how the pathogen controls the apoptotic program of the host cell will be investigated. We expect to unravel how the endoplasmic reticulum and the mitochondria are modified during infection with Simkania and how this modification contributes to the establishment and maintenance of the SCV.

衣原体目的专性胞内细菌如何在宿主细胞中建立和维持其复制泡，在很大程度上仍然是未知的。Simkania negevensis属于这组细菌，与致病性肺炎衣原体和沙眼衣原体密切相关。Simkania感染哺乳动物细胞和变形虫，因此代表了衣原体的致病性和环境成员之间几乎没有特征的进化中间体。我们最近的数据表明，Simkania在一个不寻常的空泡中复制，该空泡位于内质网内，并与宿主线粒体紧密相连。在这里，我们调查的Simkania包含液泡（SCV）的住宿的分子机制。我们将探讨SCV的形成和运输以及与宿主内质网和线粒体的相互作用。此外，将研究病原体如何控制宿主细胞的凋亡程序的基础。我们希望解开的内质网和线粒体是如何修改感染Simkania和这种修改如何有助于建立和维护的SCV。