Isotope studies of microbial sulfate reduction

该提案要求资金支持硫酸盐还原生物对S同位素分馏的实验研究，将已建立的测量硫的四种稳定同位素分馏的方法与涉及17O同位素标记技术的实验相结合。该项目旨在探索这些生物对温度和电子供体的反应。这项工作将涉及在提交提案之前进行的培养实验样品的硫同位素分析，作为研究硫酸盐还原代谢的一个更大项目的第一部分。预计这项支持将导致发表一篇关于在两种温度条件下，自养Desulfobacterium autotrophicum对电子供体变化的代谢反应的论文。了解沉积硫旋回和微生物的催化作用是了解全球碳循环的基础。该提案将为这项工作提供部分支持，为一名研究生提供几个月的支持，并为分析费用提供部分支持。

This proposal requests funds to support an experimental study of S isotope fractionation by sulfate reducing organisms, coupling well-established methods for measuring the fractionation of four stable isotopes of sulfur with experiments involving 17O isotope-labeling techniques. The project aims to explore the response of these organisms to temperature and electron donors. The work will involve sulfur isotope analysis of samples from culture experiments undertaken prior to submission of the proposal as part of the first part of a significantly larger project to study the sulfate reduction metabolism. It is anticipated that the support will lead to the publication of a paper on the metabolic response of Desulfobacterium autotrophicum to changes in the electron donor at two temperature conditions.Understanding the sedimentary sulfur cyle and the catalyses by mircobes is fundamental for understanding the global carbon cycle. The proposal will provide partial support for this work in the form of several months of support for a graduate student and partial support for analytical costs.