Engineering Angiogenesis by the Extracellular Matrix

通过细胞外基质工程血管生成

• 批准号: 1033660
• 负责人: Alvin Yeh
• 金额: $ 59.99万
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1033660&HistoricalAwards=false
• 关键词: Engineering; Angiogenesis; Extracellular; Matrix

1033660YehThis project integrates advanced microscopy with a novel bioreactor for elucidating molecular, cellular and extracellular matrix interactions that guide angiogenic morphogenesis within a 3D tissue model. Though biologically motivated, implicit in this project is further characterization of nonlinear optical phenomena induced by ultrashort optical pulses an order of magnitude shorter than what is typically used in multiphoton microscopy and demonstration of its distinct advantages in live cell NLOM for the quantitative characterization of complex biological systems. The developed biaxial bioreactor will enable precise control of the mechanical environment in which 3D models of angiogenesis are cultured. Its strengths include the ability to control matrix density, stiffness and orientation. Combined with intravital NLOM, it will provide a powerful means to decipher dynamic cell-matrix interactions in culture and to gain new insight into mechanisms that enhance or inhibit angiogenesis.

1033660 Yeh该项目将先进的显微镜技术与新型生物反应器相结合，用于阐明分子、细胞和细胞外基质的相互作用，从而在3D组织模型中指导血管生成形态发生。虽然生物动机，隐含在这个项目是进一步表征的非线性光学现象所引起的超短光脉冲的数量级短于通常用于多光子显微镜和演示其独特的优势，在活细胞NLOM复杂的生物系统的定量表征。开发的双轴生物反应器将能够精确控制培养血管生成3D模型的机械环境。其优势包括控制基质密度、刚度和方向的能力。结合活体NLOM，它将提供一个强大的手段来破译动态细胞-基质相互作用的文化，并获得新的洞察机制，增强或抑制血管生成。