Systemic phloem signals for compatibility or defence in response to fungal infections in Zea mays and Hordeum vulgare

玉米和大麦中针对真菌感染的相容性或防御的系统韧皮部信号

• 批准号: 19984681
• 负责人: Professor Dr. Aart J.E. van Bel
• 金额: --
• 依托单位: Institut für Botanik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19984681?language=en
• 关键词: Systemic; phloem; signals; compatibility; defence

Goal of this project is to catch phloem-borne signals responsible for systemic induced susceptibility (SIS) and metabolic redirection upon pathogen attack. Direct collection of phloem samples may be one promising approach to find these unknown compounds. Based on literature data and a series of preliminary experiments, we reached the conclusion that calcium may be a neglected essential element in longdistance signalling. In this study, the yield of micro-sampling of phloem sap will be improved and the collection methods adapted to the pathosystems. The phloem sap will be screened for shifts in composition between infected and non-infected compatible plant lines. Plant/fungus systems will be Zea mays/Ustilago maydis, Zea mays/Colletotrichum graminicola, Hordeum vulgare/ Blumeria graminis and Hordeum vulgare/Piriformospora indica. In the chase for candidate signals, we will first focus on Ca2+-conjugates and jasmonic acid and its relatives. In a second wave of analyses, sugars, oxygen radicals and the redox state of the sieve tube sap will be tested. The last analytical part will include the detection of macromolecules such as proteins, protein complexes and several RNA-forms. With the techniques at hand, we may be able to trace compounds responsible for both compatibility and resistance by the same set of experiments.

该项目的目标是捕获韧皮部传播的信号，这些信号负责病原体攻击时的系统诱导敏感性（SIS）和代谢重定向。直接收集韧皮部样本可能是寻找这些未知化合物的一种有前途的方法。根据文献数据和一系列初步实验，我们得出结论：钙可能是长距离信号传递中被忽视的必需元素。在本研究中，韧皮部汁液微量取样的产量将得到提高，并且收集方法将适应病理系统。将筛选韧皮部汁液以了解受感染和未受感染的相容植物系之间的成分变化。植物/真菌系统将是玉米/黑粉菌、玉米/炭疽菌、大麦/Blumeria graminis 和大麦/Piriformospora indica。在寻找候选信号时，我们将首先关注 Ca2+-缀合物和茉莉酸及其相关物。在第二波分析中，将测试糖、氧自由基和筛管汁液的氧化还原状态。最后的分析部分将包括大分子的检测，例如蛋白质、蛋白质复合物和几种 RNA 形式。借助现有的技术，我们也许能够通过同一组实验来追踪负责相容性和耐药性的化合物。