Shifts in plant gene expression and corresponding phloem sap components during trilateral interaction of barley (Hordeum vulgare), aphid species (Rhopalosiphum padi) and the aphid-transmitted Barley Yellow Dwarf Virus (BYDV)

大麦（Hordeum vulgare）、蚜虫（Rhopalosiphum padi）和蚜虫传播的大麦黄矮病毒（BYDV）三边相互作用期间植物基因表达和相应韧皮部汁液成分的变化

• 批准号: 34527299
• 负责人: Professor Dr. Aart J.E. van Bel
• 金额: --
• 依托单位: Institut für Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34527299?language=en
• 关键词: Shifts; plant; gene; expression; corresponding

The aphid-transmitted barley yellow dwarf (BYDV) luteovirus as well as the aphid-BYDV-interaction are both well described. The aphid-host and BYDV-host interactions in this tripartite relationship should be investigated before successful strategies against BYDV can be developed. The focus of this research is on the phloem: (1) aphids exclusively feed on the phloem, (2) BYDV propagation and transport is limited to the phloem, and (3) systemic responses to infestation and infection may be translocated via the sieve tubes. Logically, genes involved in barley tolerance against aphid infestation and BYDV infection are expressed in the phloem and some of their products translocated via sieve tubes. Gene and protein expression profiling are envisaged to detect local and systemic responses of aphid- and BYDV-tolerant barley cultivars. For that purpose, microarray hybridisation of leaf tissues and RNA and protein analysis of phloem exudates will be employed to identify shifts in gene and protein expression levels. Once candidate genes and proteins will have been identified, expression of the genes engaged in local resp. systemic response will be investigated by quantitative Real-Time-PCR. Subsequently, expression in local and systemic sites (phloem parenchyma, companion cells, sieve elements) will be compared. Finally, candidate genes for BYDV-tolerance will be mapped to detect co-localisation with known genetic loci (QTL) related to BYDV-tolerance. Expectedly, this study will provide a better understanding of the host response to aphid-infestation and infection by aphid-transmitted BYDV, in particular, with regard to the phloem-translocated signals of tolerant vs. susceptible host genotypes.

蚜虫传播的大麦黄矮病毒（BYDV）黄症病毒以及蚜虫-BYDV相互作用都有很好的描述。蚜虫-宿主和BYDV-宿主之间的相互作用在这种三方关系中应该被研究，然后才能开发出成功的抗BYDV策略。本研究的重点是韧皮部：（1）蚜虫专门以韧皮部为食，（2）BYDV的传播和运输仅限于韧皮部，（3）对侵染和感染的系统反应可能通过筛管转移。从逻辑上讲，大麦抗蚜虫和BYDV感染的基因在韧皮部中表达，它们的一些产物通过筛管转运。基因和蛋白质表达谱的设想，以检测本地和系统的反应蚜虫和大麦黄矮病的耐受品种。为此，将采用叶组织的微阵列杂交和韧皮部分泌物的RNA和蛋白质分析来鉴定基因和蛋白质表达水平的变化。一旦候选基因和蛋白质被确定，基因的表达将参与局部反应。将通过定量实时PCR研究全身反应。随后，将比较局部和全身部位（韧皮部薄壁组织、伴胞、筛元件）中的表达。最后，将对BYDV耐受性的候选基因进行定位，以检测与已知的BYDV耐受性相关的遗传位点（QTL）的共定位。预计，这项研究将提供一个更好的理解蚜虫感染和蚜虫传播的BYDV感染的主机响应，特别是关于耐受与敏感主机基因型的韧皮部易位信号。