MRI: Acquisition of Nikon A1RSi Confocal Microscope with Hybrid Scanner, Spectral Unmixing and FLIM/FCS

达特茅斯学院的这项重大研究仪器奖资助购买了一台带有混合扫描仪、光谱分解和允许荧光寿命成像(FLIM)和荧光相关光谱(FCS)测量的组件的共焦显微镜。该系统将极大地推进达特茅斯以及整个新汉普郡和佛蒙特州的基础科学研究和教学基础设施。由新系统实现的研究解决了细胞生物学和发育生物学中的基本问题，并利用了一系列不同的模型系统(细菌、真菌、藻类、植物、蠕虫、苍蝇和老鼠)。在所有情况下，新的共焦系统都将大幅改进和扩大成像能力，使达特茅斯研究人员能够继续进行前沿研究，并培训下一代生命科学家。每一位使用新型显微镜的教员都致力于教授所有级别的科学，并培养成功的未来科学家和科学教师。新系统使最先进的成像技术更容易为本科生、研究生和博士后学者以及我们当地中学的学生和教师所使用。此外，达特茅斯大学的教职员工在将本科生实习生纳入他们的研究计划以及将最新的科学方法和数据纳入他们的课程方面有着广泛的历史。这些研究和教学工作的结果将通过摘要和同行评议的出版物以及学生和教职员工积极参加专业会议的方式广泛传播。

This Major Research Instrumentation award to Dartmouth College funds the acquisition of a confocal microscope with a hybrid scanner, spectral unmixing and components that allow for Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS) measurements. This system will significantly advance basic science research and teaching infrastructure at Dartmouth and throughout New Hampshire and Vermont. The research enabled by the new system addresses fundamental questions in cell biology and developmental biology, and utilizes a diverse array of model systems (bacteria, fungi, algae, plants, worms, flies and mice). In all cases, the new confocal system will substantially improve and expand the imaging capabilities and allow Dartmouth investigators to continue to conduct leading-edge research and to train the next generation of life scientists. Each of the faculty using the new microscope is committed to teaching science at all levels and to preparing successful future scientists and science teachers. The new system makes state-of-the-art imaging technology more accessible to undergraduate students, graduate students and post-doctoral scholars, as well as students and teachers in our local secondary schools. Furthermore, Dartmouth faculty members have an extensive history of including undergraduate interns in their research programs and incorporating the latest scientific approaches and data into their courses. The results of these research and teaching efforts will be broadly disseminated through abstracts and peer reviewed publications, as well as by active participation of students and faculty at professional meetings.