Dimensions: Collaborative Research: Deconstructing diversity and ecosystem function at multiple spatial and genetic scales in a keystone plant-microbe symbiosis

维度：合作研究：在关键的植物-微生物共生中解构多个空间和遗传尺度的多样性和生态系统功能

• 批准号: 1046115
• 负责人: Thomas Bruns
• 金额: $ 71.75万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1046115&HistoricalAwards=false
• 关键词: Dimensions; Collaborative; Research; Deconstructing; diversity

This work will provide the first continental-scale sample of taxonomic, genetic and functional diversity of ectomycorrhizal fungi from pine ecosystems. These fungi form symbiotic relationships with the roots of dominant forest plants, and provide them with nutrients and water in exchange for photosynthetically-fixed carbon. Despite the importance of ectomycorrhizal fungi in global nutrient cycles, little is known about large-scale patterns of fungal community structure relative to plants, animals, or even bacteria. This project will establish patterns of gene flow and selection for multiple ectomycorrhizal fungi across North America, use a hierarchical phylogenetic sampling design to measure variation in functional enzyme production across individuals, populations and species, and use RNA based metagenomics to measure functional trait expression on mycorrhizal root tips. The project will result in a large, open access database of taxonomic barcode and functional genes that can be used by researchers to generate predictive models of fungal ecosystems. It will include training of graduate students and postdoctoral scholars in cutting-edge techniques in an understudied field, will result in outreach activities targeted toward the general public, and will include activities for K-12 students and educators designed to foster early student interests in fungi.

这项工作将提供第一个来自松树生态系统外生霉素真菌分类，遗传和功能多样性的大陆规模样本。这些真菌与主要的森林植物的根部形成共生关系，并为它们提供营养和水，以换取光合固定的碳。尽管在全球营养周期中，外生菌血真菌的重要性很重要，但对于相对于植物，动物甚至细菌，对真菌群落结构的大规模模式知之甚少。该项目将建立北美多种外生菌的真菌的基因流量和选择模式，使用分层系统发育采样设计来衡量个体，人群和物种之间功能性酶产生的变化，并使用基于RNA的基因组学来测量mycorrhizal root tip上的功能性状表达。该项目将导致一个大型开放访问数据库的分类条形码和功能基因的数据库，研究人员可以将其用于生成真菌生态系统的预测模型。它将包括对研究生的研究生和博士后学者的培训，在研究研究的领域中，将导致针对公众的外展活动，并将包括针对旨在促进真菌早期学生利益的K-12学生和教育工作者的活动。