Functional Traits of Key Species in the Ectomycorrhizal Community: Genet Size and Colonization Strategy

外生菌根群落关键物种的功能特征：基因大小和定植策略

• 批准号: 9815262
• 负责人: Thomas Bruns
• 金额: $ 35万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9815262&HistoricalAwards=false
• 关键词: Functional; Traits; Key; Species; Ectomycorrhizal

9815262BrunsEctomycorrhizal fungi (EM) are obligate symbionts of most of the dominant tree species in temperate forest ecosystems. These fungi have been shown to be necessary for the growth and development of fine roots in many systems. It is in the fine roots, where most plants get many of the nutrients they need and the fungi play a vital role in the development of these roots and may also may have a direct role in the transfer of nutrients. In spite of their importance, EM fungi are often neglected by plant ecologists or they are treated as a single group (without regard to species). Due to their small size, the difficulty of and time consuming methods of species identification, coupled with their belowground habitat, working with them especially at the species level has difficult in the past. This project will determine the colonization strategy of five species of EM fungi in a temperate forest ecosystem. Specifically, this proposal would study the sizes and spatial patterning for genets of 3 of the 5 species that have very different life strategies. A molecular technique (Amplified Fragment Length Polymorphisms) will be used as the primary tool for discerning genetic identity. This technique will provide a fast, reliable and abundant source of variable markers, which will all allow the PI to identify the EM fungi to the species level. In addition, using this technique, a better understanding of the spatial patterns of EM fungi will be done. This work will provide tools for future investigators and allow them to explore and understand the population structure of key EM fungi.

9815262 Brunscontomycorrhizal真菌（EM）是温带森林生态系统中大多数主要树种的强制性共生体。这些真菌已被证明是许多系统中细根的生长和发展所必需的。正是在细根中，大多数植物都能获得所需的许多营养，而真菌在这些根源的发展中起着至关重要的作用，并且可能在养分的转移中也可能直接作用。 尽管具有重要性，但EM真菌经常被植物生态学家忽略，或者被视为一组（不考虑物种）。由于它们的尺寸很小，物种识别的难度和耗时方法，再加上其地下栖息地，尤其是在物种水平上与之合作。该项目将确定温带森林生态系统中五种EM真菌的殖民策略。具体而言，该提案将研究具有截然不同的生活策略的5种物种中的3种基因的大小和空间图案。 分子技术（放大的片段长度多态性）将用作辨别遗传认同的主要工具。该技术将提供可变标记的快速，可靠和丰富的来源，这都可以使PI识别出EM真菌到物种水平。此外，使用此技术，将对EM真菌的空间模式有更好的了解。这项工作将为未来的调查人员提供工具，并允许他们探索和了解关键EM真菌的种群结构。