SBIR Phase I: Enzyme Assisted Pulping: Feasibility of Using Enzymes to Break Non-Glycosidic Ether Bonds between Xylan and Lignin

SBIR 第一阶段：酶辅助制浆：使用酶破坏木聚糖和木质素之间非糖苷醚键的可行性

• 批准号: 1046844
• 负责人: Nancy Kravit
• 金额: $ 15万
• 依托单位: Tethys Research LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1046844&HistoricalAwards=false
• 关键词: SBIR; Phase; Enzyme; Assisted; Pulping

This Small Business Innovation Research (SBIR) Phase I project helps unlock the potential of forests to provide sustainable, carbon-neutral raw material for much of the nation?s energy needs. Tethys will search for enzymes specific for ether bonds between lignin and the hardwood hemicellulose, xylan. A fluorogenic model of xylan-lignin ether bonds will be synthesized that fluoresces when the xylan-lignin ether bond is broken. It will be used to bioprospect for xylan lignin etherase (XLE) activity in culture collections and sites of hardwood decay in Maine forests. Putative positives will be tested for autofluorescence, XLE solubility, and XLE cofactor requirement. Results of this project will advance understanding of etherase enzymatic mechanisms, increase knowledge about the chemistry of sugar-lignin ether bonds, and contribute to the understanding of wood decay. The broader/commercial impacts of this research are two-fold. First, development of a novel, environmentally friendly process for pulp products becomes possible. The new process will also create new feedstocks for biofuels and platform chemicals. Second, the development of the new method means that (i) wood can be used to meet a significant portion of America?s energy needs; (ii) corn slated for ethanol production can be directed to food products; (iii) energy and industrial chemicals used in pulp and paper mills will be reduced and (iv) America?s pulp and paper industry (and the rural towns where mills are located) will receive a much needed economic boost from reduced costs and increased revenues from valuable new products.

这个小企业创新研究（SBIR）第一阶段项目有助于释放森林的潜力，为全国大部分地区提供可持续的碳中和原材料？的能源需求。特提斯公司将寻找对木质素和硬木半纤维素，木聚糖之间的醚键有特异性的酶。将合成木聚糖-木质素醚键的荧光模型，当木聚糖-木质素醚键断裂时，该模型发荧光。它将被用来生物勘探木聚糖木质素醚酶（XLE）的活动，在文化收集和网站的硬木腐烂在缅因州森林。将检测推定阳性的自发荧光、XLE溶解度和XLE辅因子要求。本计画的结果将增进对醚酶机制的了解，增加关于糖-木质素醚键化学的知识，并有助于了解木材腐烂。这项研究的更广泛/商业影响是双重的。首先，开发一种新的、环境友好的纸浆产品工艺成为可能。新工艺还将为生物燃料和平台化学品创造新的原料。第二，新方法的发展意味着（i）木材可以用来满足美国的很大一部分？能源需求;（二）玉米预定用于乙醇生产可以直接用于食品;（三）能源和工业化学品用于纸浆和造纸米尔斯将减少和（四）美国？美国的纸浆和造纸工业（以及米尔斯工厂所在的农村城镇）将从降低成本和增加有价值的新产品的收入中获得急需的经济增长。