Acquisition, Assembly and Use of a High Speed Fluorescence Anisotropy and Lifetime Imaging System

高速荧光各向异性和寿命成像系统的采集、组装和使用

• 批准号: 1048548
• 负责人: John McCracken
• 金额: $ 31.14万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-12-15 至 2013-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1048548&HistoricalAwards=false
• 关键词: Acquisition; Assembly; Use; Speed; Fluorescence

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor John McCracken and colleagues Gary Blanchard, Robert Ofoli, Greg Swain and David Weliky from Michigan State University will acquire a series of components to build a rapid acquisition, picosecond fluorescence lifetime and anisotropy imaging system. The award will enhance research training and education at all levels, especially in areas of study such as (a) fluorescence lifetime and anisotropy imaging of lipid structures, (b) optical and electrochemical characterization of biomimetic nanostructured interfaces, (c) chemically modified electrodes for studies of graphene and thin carbon films, and (d) imaging of membrane perturbations induced by viral fusion peptides and proteins.A rapid acquisition, picosecond fluorescence lifetime and anisotropy imaging system is important to the study of a broad range of structurally heterogeneous and fluid interfaces such as those in biological membranes. These experiments provide novel information on molecular motion and energy transfer, and specifically how these properties vary with the physical condition and chemical composition of the interface. This instrumentation will support not only research activities but also research training to graduate and undergraduate students at Michigan State University and nearby institutions such as Saginaw Valley State University. The instrument will also support international interactions with the University of Warsaw, University of Bath and Shaanxi Normal University as well as activities with local high school students.

凭借化学研究仪器和设施：多用户 (CRIF:MU) 计划的这一奖项，密歇根州立大学的 John McCracken 教授及其同事 Gary Blanchard、Robert Ofoli、Greg Swain 和 David Weliky 将获得一系列组件，以构建快速采集、皮秒荧光寿命和各向异性成像系统。该奖项将加强各级研究培训和教育，特别是在以下研究领域：（a）脂质结构的荧光寿命和各向异性成像，（b）仿生纳米结构界面的光学和电化学表征，（c）用于石墨烯和碳薄膜研究的化学修饰电极，以及（d）病毒融合肽和病毒引起的膜扰动的成像 快速采集、皮秒荧光寿命和各向异性成像系统对于研究广泛的结构异质和流体界面（例如生物膜中的界面）非常重要。这些实验提供了有关分子运动和能量转移的新信息，特别是这些特性如何随界面的物理条件和化学成分而变化。该仪器不仅支持研究活动，还支持密歇根州立大学和萨吉诺谷州立大学等附近机构的研究生和本科生的研究培训。该工具还将支持与华沙大学、巴斯大学和陕西师范大学的国际互动以及与当地高中生的活动。