Cellular and molecular dynamics of cell-cell contact formation in the context of directional germ cell migration during Xenopus embryogenesis

爪蟾胚胎发生过程中定向生殖细胞迁移背景下细胞接触形成的细胞和分子动力学

• 批准号: 200513125
• 负责人: Professor Dr. Andreas Janshoff
• 金额: --
• 依托单位: Institut für Physikalische Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200513125?language=en
• 关键词: Cellular; molecular; dynamics; cell; contact

Germ cell development in Xenopus embryos is regulated by maternal determinants such as vegetally localizing mRNAs, which get associated with the germ plasm during early oogenesis. Some of the proteins encoded by these mRNAs were demonstrated to serve functions in the context of the direc-tional migration of the primordial germ cells (PGCs). Within the endodermal germ layer, PGCs first migrate passively with the gastrulation movements, before they lose contact with each other and start directional migration during tailbud stages of development. This transition from passive to active migration is the subject of this proposal. In particular, we aim to analyze the regulatory role of maternal factors in respect to the formation of cell-cell contacts, as well as for the cellular dynamics by use of biophysical techniques.

非洲爪蟾胚胎中的生殖细胞发育受母体决定因素如植物定位mRNA的调节，这些决定因素在早期卵子发生期间与生殖质相关。由这些mRNA编码的一些蛋白质被证明在原始生殖细胞（PGCs）的定向迁移的背景下起作用。在内胚层内，PGCs首先随着原肠胚形成运动被动地迁移，然后它们彼此失去联系，并在发育的尾芽阶段开始定向迁移。从被动迁移到主动迁移的转变是本提案的主题。特别是，我们的目标是分析母源性因素在细胞-细胞接触形成方面的调节作用，以及通过使用生物物理技术的细胞动力学。