Molecular and cellular basis of mosquito olfactory attraction to hay infusion for enhanced vector surveillance and control
蚊子对干草浸液的嗅觉吸引力的分子和细胞基础,以加强病媒监测和控制
基本信息
- 批准号:10648584
- 负责人:
- 金额:$ 24.12万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-01-18 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:AdhesivesAedesAfferent NeuronsAfricanAnalytical ChemistryArbovirusesBehaviorBehavioralBehavioral AssayBiological AssayBloodBotanicalsCessation of lifeChemicalsChemoreceptorsConstitutionConstitutionalCulicidaeCynodonDataDengueDesiccationDevelopmentDisease VectorsEngineeringFemaleFermentationFormulationFutureGeneticGoalsGrantGravidHeterozygoteHumanInfusion proceduresLaboratoriesLigandsMediatingMethodsMolecularMolecular TargetNeuronsOlfactory PathwaysOvipositionPartner in relationshipPathway interactionsPhysiologicalPlayPopulationPopulation ControlPopulation DynamicsPopulation SurveillancePuerto RicoResearchResistanceReverse engineeringRoleSearching BehaviorSensorySeriesSiteSourceTestingWaterYellow FeverZIKAbehavior testcell typeeggfeedingfield studyimprovedinsightloss of functionmanmutantneuralneural circuitneurophysiologynovelolfactory sensory neuronspreferencepregnantreceptorresponsetoolurban settingvectorvolatile organic compound
项目摘要
The mosquito Aedes aegypti is a prolific disease vector that thrives in urban environments. Its explosive
population dynamics are driven by the ability of female Ae. aegypti to seek out standing pools of water to lay
their desiccation-resistant eggs. Of note, females are attracted to the scent of decomposing botanical matter in
water, but only after mating and blood-feeding. In particular, gravid female Ae. aegypti are highly attracted to
the scent of fermenting African Bermuda grass hay in water, which has been exploited for mosquito trapping
strategies in the field that aim to lure this major disease vector to its death during the act of egg laying. We
propose to apply integrative methods to define the molecular and cellular basis of Ae. aegypti olfactory
attraction to hay infusion which will catalyze identification of olfactory circuitry mediating oviposition site search
and novel oviposition attractants for this disease vector. To elucidate candidate chemoreceptors and
associated neurons implicated in mosquito olfactory attraction to hay infusion, we will test the role of different
chemoreceptor classes using loss-of-function genetics and olfactory sensory neuron populations by selectively
disrupting neural activity using cell-type specific neuronal silencing. In parallel, we aim to reverse engineer
attractive blends of oviposition attractants mimicking the scent of hay infusion by leveraging chemical analysis
of infusion headspace combined with neural activity assays that compare olfactory sensitivity and response
amplitudes of non-gravid and gravid mosquitoes to conserved volatile organic compounds found in this this
natural lure. These aims will fundamentally improve our understanding of the chemosensory basis of Ae.
aegypti oviposition site search behavior. From a translational perspective, this research will reveal new
molecular targets in the Ae. aegypti olfactory system to modulate mosquito egg laying behavior and identify
novel synthetic formulations of oviposition attractants for enhanced population surveillance and control of this
globally important disease vector.
埃及伊蚊是一种多产的疾病媒介,在城市环境中蓬勃发展。它的爆炸性
种群动态是由雌性Ae的能力驱动的。埃及人寻找站立的水池来铺设
它们耐脱水的卵。值得注意的是,雌性被植物分解物质的气味所吸引
水,但只有在交配和吸血之后。特别是怀孕的雌性Ae.埃及伊蚊被高度吸引
在水中发酵的非洲百慕大干草的气味,已被用来诱蚊
旨在引诱这种主要病媒在产卵过程中死亡的田间策略。我们
建议应用综合方法确定Ae的分子和细胞基础。埃及伊蚊嗅觉
对干草输注的吸引力将催化识别介导产卵地点搜索的嗅觉回路
以及针对该病媒的新型产卵引诱剂。阐明候选化学感受器和
相关神经元牵涉到蚊子嗅觉吸引干草的输注,我们将测试不同的作用
选择性使用功能丧失遗传学和嗅觉神经元群体的化学感受器分类
通过细胞类型的特定神经元沉默来扰乱神经活动。同时,我们的目标是反向工程
利用化学分析模拟干草浸泡的气味的产卵诱导剂的诱人混合物
与神经活动测试相结合,比较嗅觉敏感性和反应
本研究中发现的非妊娠和妊娠蚊子对保守的挥发性有机化合物的幅度
天然的诱饵。这些目的将从根本上提高我们对Ae的化学感觉基础的理解。
埃及斑潜蝇产卵地点搜索行为。从翻译的角度来看,这项研究将揭示出新的
Ae中的分子靶标。埃及伊蚊嗅觉系统对蚊子产卵行为的调控和识别
用于加强种群监测和控制的产卵引诱剂的新合成配方
全球重要的疾病媒介。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Conor James McMeniman其他文献
Conor James McMeniman的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Conor James McMeniman', 18)}}的其他基金
Molecular Architecture of the Mosquito Carbon Dioxide Receptor Complex
蚊子二氧化碳受体复合物的分子结构
- 批准号:
9808786 - 财政年份:2019
- 资助金额:
$ 24.12万 - 项目类别:
相似海外基金
Defining the antiviral effects of Wolbachia in Aedes aegypti mosquitoes
确定沃尔巴克氏体对埃及伊蚊的抗病毒作用
- 批准号:
DP220102997 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
Discovery Projects
Molecular and functional characterization of olfactory pathways in the arbovirus vector mosquito Aedes aegypti
虫媒病毒载体蚊子埃及伊蚊嗅觉通路的分子和功能特征
- 批准号:
10638710 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
The molecular basis of fatty acid taste in Aedes aegypti
埃及伊蚊脂肪酸味道的分子基础
- 批准号:
10679953 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
Genomic Analysis of Aedes aegypti Host Preference Across Urban-Rural Gradients in Africa
非洲城乡梯度埃及伊蚊寄主偏好的基因组分析
- 批准号:
10525807 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
Collaborative Research: Olfactory learning and neuromodulation in the Aedes aegypti mosquito
合作研究:埃及伊蚊的嗅觉学习和神经调节
- 批准号:
2242603 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
Standard Grant
Collaborative Research: Olfactory learning and neuromodulation in the Aedes aegypti mosquito
合作研究:埃及伊蚊的嗅觉学习和神经调节
- 批准号:
2242604 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
Standard Grant
Exploring the coevolutionary potential of chikungunya virus and its Aedes mosquito vectors
探索基孔肯雅病毒及其伊蚊媒介的共同进化潜力
- 批准号:
10711906 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
The development of biorational pesticides targeting lncRNAs in adult female Aedes aegypti mosquitoes
针对成年雌性埃及伊蚊 lncRNA 的生物合理农药的开发
- 批准号:
10742558 - 财政年份:2023
- 资助金额:
$ 24.12万 - 项目类别:
Developing novel genetic and symbiotic control strategies for the invasive mosquito, Aedes japonicus
为入侵性蚊子日本伊蚊开发新型遗传和共生控制策略
- 批准号:
BB/W018446/1 - 财政年份:2022
- 资助金额:
$ 24.12万 - 项目类别:
Research Grant
Doctoral Dissertation Research: Phylogeographic Investigation of Aedes aegypti
博士论文研究:埃及伊蚊的系统发育地理学调查
- 批准号:
2210879 - 财政年份:2022
- 资助金额:
$ 24.12万 - 项目类别:
Standard Grant