Molecular and cellular basis of mosquito olfactory attraction to hay infusion for enhanced vector surveillance and control
蚊子对干草浸液的嗅觉吸引力的分子和细胞基础,以加强病媒监测和控制
基本信息
- 批准号:10648584
- 负责人:
- 金额:$ 24.12万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-01-18 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:AdhesivesAedesAfferent NeuronsAfricanAnalytical ChemistryArbovirusesBehaviorBehavioralBehavioral AssayBiological AssayBloodBotanicalsCessation of lifeChemicalsChemoreceptorsConstitutionConstitutionalCulicidaeCynodonDataDengueDesiccationDevelopmentDisease VectorsEngineeringFemaleFermentationFormulationFutureGeneticGoalsGrantGravidHeterozygoteHumanInfusion proceduresLaboratoriesLigandsMediatingMethodsMolecularMolecular TargetNeuronsOlfactory PathwaysOvipositionPartner in relationshipPathway interactionsPhysiologicalPlayPopulationPopulation ControlPopulation DynamicsPopulation SurveillancePuerto RicoResearchResistanceReverse engineeringRoleSearching BehaviorSensorySeriesSiteSourceTestingWaterYellow FeverZIKAbehavior testcell typeeggfeedingfield studyimprovedinsightloss of functionmanmutantneuralneural circuitneurophysiologynovelolfactory sensory neuronspreferencepregnantreceptorresponsetoolurban settingvectorvolatile organic compound
项目摘要
The mosquito Aedes aegypti is a prolific disease vector that thrives in urban environments. Its explosive
population dynamics are driven by the ability of female Ae. aegypti to seek out standing pools of water to lay
their desiccation-resistant eggs. Of note, females are attracted to the scent of decomposing botanical matter in
water, but only after mating and blood-feeding. In particular, gravid female Ae. aegypti are highly attracted to
the scent of fermenting African Bermuda grass hay in water, which has been exploited for mosquito trapping
strategies in the field that aim to lure this major disease vector to its death during the act of egg laying. We
propose to apply integrative methods to define the molecular and cellular basis of Ae. aegypti olfactory
attraction to hay infusion which will catalyze identification of olfactory circuitry mediating oviposition site search
and novel oviposition attractants for this disease vector. To elucidate candidate chemoreceptors and
associated neurons implicated in mosquito olfactory attraction to hay infusion, we will test the role of different
chemoreceptor classes using loss-of-function genetics and olfactory sensory neuron populations by selectively
disrupting neural activity using cell-type specific neuronal silencing. In parallel, we aim to reverse engineer
attractive blends of oviposition attractants mimicking the scent of hay infusion by leveraging chemical analysis
of infusion headspace combined with neural activity assays that compare olfactory sensitivity and response
amplitudes of non-gravid and gravid mosquitoes to conserved volatile organic compounds found in this this
natural lure. These aims will fundamentally improve our understanding of the chemosensory basis of Ae.
aegypti oviposition site search behavior. From a translational perspective, this research will reveal new
molecular targets in the Ae. aegypti olfactory system to modulate mosquito egg laying behavior and identify
novel synthetic formulations of oviposition attractants for enhanced population surveillance and control of this
globally important disease vector.
埃及伊蚊是一种在城市环境中大量繁殖的疾病媒介。其爆炸式
种群动态由雌性Ae的能力驱动。埃及人寻找水池,
它们的抗干燥卵值得注意的是,雌性会被腐烂的植物气味所吸引,
水,但只有在交配和吸血后。特别是怀孕的雌性Ae.埃及人非常喜欢
非洲百慕大干草在水中发酵的气味,这种气味被用来诱捕蚊子
该领域的战略旨在引诱这种主要疾病媒介在产卵过程中死亡。我们
建议应用综合方法来确定Ae的分子和细胞基础。埃及嗅觉
对干草浸渍液的吸引力,这将催化识别介导产卵地点搜索的嗅觉回路
以及该疾病媒介的新产卵引诱剂。为了阐明候选化学感受器,
相关神经元牵连在蚊子嗅觉吸引干草输液,我们将测试不同的作用
化学感受器类别使用功能丧失遗传学和嗅觉感觉神经元群体通过选择性
使用细胞类型特异性神经元沉默破坏神经活性。与此同时,我们的目标是逆向工程,
通过利用化学分析模仿干草浸液气味的产卵引诱剂的有吸引力的混合物
结合神经活动测定,比较嗅觉灵敏度和反应
振幅的非妊娠和妊娠蚊子保守的挥发性有机化合物中发现,这
天然的诱惑这些目标将从根本上提高我们对Ae的化学感受基础的理解。
埃及伊蚊产卵场所搜索行为从翻译的角度来看,这项研究将揭示新的
Ae中的分子靶点。埃及伊蚊嗅觉系统调节蚊子产卵行为和识别
用于增强种群监视和控制这种害虫的产卵引诱剂的新合成制剂
全球重要的病媒。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Conor James McMeniman其他文献
Conor James McMeniman的其他文献
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{{ truncateString('Conor James McMeniman', 18)}}的其他基金
Molecular Architecture of the Mosquito Carbon Dioxide Receptor Complex
蚊子二氧化碳受体复合物的分子结构
- 批准号:
9808786 - 财政年份:2019
- 资助金额:
$ 24.12万 - 项目类别:
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