Molecular and cellular basis of mosquito olfactory attraction to hay infusion for enhanced vector surveillance and control

蚊子对干草浸液的嗅觉吸引力的分子和细胞基础，以加强病媒监测和控制

• 批准号: 10648584
• 负责人: Conor James McMeniman
• 金额: $ 24.12万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-18 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10648584
• 关键词: Adhesives; Aedes; Afferent Neurons; African; Analytical Chemistry; Arboviruses; Behavior; Behavioral; Behavioral Assay; Biological Assay; Blood; Botanicals; Cessation of life; Chemicals; Chemoreceptors; Constitution; Constitutional; Culicidae; Cynodon; Data; Dengue; Desiccation; Development; Disease Vectors; Engineering; Female; Fermentation; Formulation; Future; Genetic; Goals; Grant; Gravid; Heterozygote; Human; Infusion procedures; Laboratories; Ligands; Mediating; Methods; Molecular; Molecular Target; Neurons; Olfactory Pathways; Oviposition; Partner in relationship; Pathway interactions; Physiological; Play; Population; Population Control; Population Dynamics; Population Surveillance; Puerto Rico; Research; Resistance; Reverse engineering; Role; Searching Behavior; Sensory; Series; Site; Source; Testing; Water; Yellow Fever; ZIKA; behavior test; cell type; egg; feeding; field study; improved; insight; loss of function; man; mutant; neural; neural circuit; neurophysiology; novel; olfactory sensory neurons; preference; pregnant; receptor; response; tool; urban setting; vector; volatile organic compound

The mosquito Aedes aegypti is a prolific disease vector that thrives in urban environments. Its explosive population dynamics are driven by the ability of female Ae. aegypti to seek out standing pools of water to lay their desiccation-resistant eggs. Of note, females are attracted to the scent of decomposing botanical matter in water, but only after mating and blood-feeding. In particular, gravid female Ae. aegypti are highly attracted to the scent of fermenting African Bermuda grass hay in water, which has been exploited for mosquito trapping strategies in the field that aim to lure this major disease vector to its death during the act of egg laying. We propose to apply integrative methods to define the molecular and cellular basis of Ae. aegypti olfactory attraction to hay infusion which will catalyze identification of olfactory circuitry mediating oviposition site search and novel oviposition attractants for this disease vector. To elucidate candidate chemoreceptors and associated neurons implicated in mosquito olfactory attraction to hay infusion, we will test the role of different chemoreceptor classes using loss-of-function genetics and olfactory sensory neuron populations by selectively disrupting neural activity using cell-type specific neuronal silencing. In parallel, we aim to reverse engineer attractive blends of oviposition attractants mimicking the scent of hay infusion by leveraging chemical analysis of infusion headspace combined with neural activity assays that compare olfactory sensitivity and response amplitudes of non-gravid and gravid mosquitoes to conserved volatile organic compounds found in this this natural lure. These aims will fundamentally improve our understanding of the chemosensory basis of Ae. aegypti oviposition site search behavior. From a translational perspective, this research will reveal new molecular targets in the Ae. aegypti olfactory system to modulate mosquito egg laying behavior and identify novel synthetic formulations of oviposition attractants for enhanced population surveillance and control of this globally important disease vector.

埃及伊蚊是一种在城市环境中大量繁殖的疾病媒介。其爆炸式 种群动态由雌性Ae的能力驱动。埃及人寻找水池， 它们的抗干燥卵值得注意的是，雌性会被腐烂的植物气味所吸引， 水，但只有在交配和吸血后。特别是怀孕的雌性Ae.埃及人非常喜欢 非洲百慕大干草在水中发酵的气味，这种气味被用来诱捕蚊子 该领域的战略旨在引诱这种主要疾病媒介在产卵过程中死亡。我们 建议应用综合方法来确定Ae的分子和细胞基础。埃及嗅觉 对干草浸渍液的吸引力，这将催化识别介导产卵地点搜索的嗅觉回路 以及该疾病媒介的新产卵引诱剂。为了阐明候选化学感受器， 相关神经元牵连在蚊子嗅觉吸引干草输液，我们将测试不同的作用 化学感受器类别使用功能丧失遗传学和嗅觉感觉神经元群体通过选择性 使用细胞类型特异性神经元沉默破坏神经活性。与此同时，我们的目标是逆向工程， 通过利用化学分析模仿干草浸液气味的产卵引诱剂的有吸引力的混合物 结合神经活动测定，比较嗅觉灵敏度和反应 振幅的非妊娠和妊娠蚊子保守的挥发性有机化合物中发现，这 天然的诱惑这些目标将从根本上提高我们对Ae的化学感受基础的理解。 埃及伊蚊产卵场所搜索行为从翻译的角度来看，这项研究将揭示新的 Ae中的分子靶点。埃及伊蚊嗅觉系统调节蚊子产卵行为和识别 用于增强种群监视和控制这种害虫的产卵引诱剂的新合成制剂 全球重要的病媒。