Reversed Isoprenoid Biosynthesis using Isoprene as an Abundant Substrate: A New Pathway to Renewable Hydrocarbon-Based Compounds and Materials

使用异戊二烯作为丰富底物的逆向类异戊二烯生物合成：可再生烃基化合物和材料的新途径

• 批准号: 1057954
• 负责人: Judit Puskas
• 金额: $ 31.5万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1057954&HistoricalAwards=false
• 关键词: Reversed; Isoprenoid; Biosynthesis; using; Isoprene

In this project funded by the Macromolecular, Supramolecular and Nanochemistry Program of the Chemistry Division, Judit Puskas of the University of Akron will develop strategies to quantify the formation of natural rubber, a polyterpenoid, from isoprene and to characterize the microstructure of the polyisoprene formed. The idea of the project is to gain a fundamental understanding of the reversible equilibria involved in terpenoid biosynthesis and to optimize their production from isoprene, an abundant substrate than can be obtained from renewable resources. The approach is to monitor the kinetics of monomer conversion in the presence of selected enzyme cocktails using high resolution size exclusion chromatography, nuclear magnetic resonance spectroscopy, and in situ infrared and Raman spectroscopy techniques. The production of natural rubber from Guayule and Russian Dandelion will also be investigated following the same strategy. The exact structure and molar mass of the obtained isoprenoids will be determined using a variety of analytical techniques, and the data will be used to reconstruct the biosynthetic reaction pathway. The project will be performed in collaboration with Alain Deffieux, University of Bordeaux, France, and Manuel Rodriguez-Concepcion, Centre for Research on Agricultural Genomics (CRAG), Spain. The broader impacts involve developing infrastructure for research and education through establishing multilateral international collaborations as well as promoting teaching, training and learning of graduate students through this project and its international dimensions. The project will encourage students to think "green," finding ways to emulate natural chemical pathways and contributing to sustainable materials development for future generations.Isoprene and terpenoids are produced by all living things: plants, microbes and animals. They have essential functions in life processes such as respiration, photosynthesis, the regulation of growth and development, and others. They are also used to make important elastic materials that are used in a range of industries, such as automotive, apparel, and medical equipment. This project will contribute to a better understanding of the biochemistry of terpenoids and lead to green processes to access these chemicals.

在这个由化学部门大分子、超分子和纳米化学项目资助的项目中，阿克伦大学的Judit Puskas将制定策略，量化由异戊二烯生成天然橡胶（一种聚萜）的过程，并表征聚异戊二烯形成的微观结构。该项目的想法是对萜类生物合成的可逆平衡有一个基本的了解，并优化异戊二烯的生产，异戊二烯是一种比可再生资源更丰富的底物。该方法是使用高分辨率粒径排阻色谱、核磁共振波谱、原位红外和拉曼光谱技术，在选定的酶鸡尾酒存在下监测单体转化动力学。根据同样的策略，还将研究用银胶菊和俄罗斯蒲公英生产天然橡胶。得到的类异戊二烯的精确结构和摩尔质量将使用各种分析技术来确定，这些数据将用于重建生物合成反应途径。该项目将与法国波尔多大学的Alain Deffieux和西班牙农业基因组学研究中心（CRAG）的Manuel Rodriguez-Concepcion合作开展。更广泛的影响包括通过建立多边国际合作发展研究和教育基础设施，以及通过该项目及其国际层面促进研究生的教学、培训和学习。该项目将鼓励学生思考“绿色”，寻找模拟自然化学途径的方法，为子孙后代的可持续材料开发做出贡献。异戊二烯和萜类化合物由所有生物产生：植物、微生物和动物。它们在生命过程中具有重要的功能，如呼吸、光合作用、生长发育调节等。它们还用于制造重要的弹性材料，这些材料用于汽车、服装和医疗设备等一系列行业。这个项目将有助于更好地了解萜类化合物的生物化学，并导致绿色过程来获取这些化学物质。