High-Resolution Secondary Ion Mass Spectrometry for Chemically Imaging Cell Membrane Organization

用于细胞膜组织化学成像的高分辨率二次离子质谱

• 批准号: 1058809
• 负责人: Mary Kraft
• 金额: $ 36万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1058809&HistoricalAwards=false
• 关键词: Resolution; Secondary; Ion; Mass; Spectrometry

With support from the Chemical Measurement and Imaging Program, Prof. Mary Kraft and her group at the University of Illinois are developing a new approach to chemical imaging of organized structures like membranes using high-resolution secondary ion mass spectrometry. The work entails development and optimization of functionalized protein-specific affinity labels, metabolic labeling strategies, cell preservation protocols, and statistical methods to evaluate component co-localization. The method will significantly impact our understanding of how plasma membrane organization affects cellular function, and the molecular interactions that produce this organization. The project will provide interdisciplinary training of graduate students. Other educational activities focus on promoting the professional development of K-12 math and science teachers and educating middle and high school students about the chemical interactions that drive biological membrane organization. Results will be incorporated into laboratory activities and supplemental teaching materials for middle and high school students that will be provided to teachers through the Nano-CEMMS Center at the University of Illinois.

在化学测量和成像计划的支持下，伊利诺伊大学的玛丽卡夫教授和她的小组正在开发一种新的方法，使用高分辨率二次离子质谱法对膜等组织结构进行化学成像。这项工作需要开发和优化功能化蛋白质特异性亲和标记，代谢标记策略，细胞保存协议和统计方法来评估组件的共定位。 该方法将显著影响我们对质膜组织如何影响细胞功能以及产生这种组织的分子相互作用的理解。该项目将为研究生提供跨学科培训。其他教育活动的重点是促进K-12数学和科学教师的专业发展，并教育初中和高中学生关于驱动生物膜组织的化学相互作用。结果将被纳入实验室活动和补充教材，初中和高中学生，将提供给教师通过纳米CEMMS中心在伊利诺伊大学。