High-Resolution Secondary Ion Mass Spectrometry for Chemically Imaging Cell Membrane Organization

在化学测量和成像项目的支持下，伊利诺伊大学的Mary Kraft教授和她的团队正在开发一种使用高分辨率二次离子质谱法对膜等组织结构进行化学成像的新方法。这项工作需要开发和优化功能化蛋白质特异性亲和标记、代谢标记策略、细胞保存协议和评估组分共定位的统计方法。该方法将显著影响我们对质膜组织如何影响细胞功能的理解，以及产生这种组织的分子相互作用。该项目将为研究生提供跨学科的培训。其他教育活动侧重于促进K-12数学和科学教师的专业发展，并教育初高中学生关于驱动生物膜组织的化学相互作用。研究结果将被纳入实验室活动和初中和高中学生的补充教材中，并通过伊利诺伊大学纳米- cemms中心提供给教师。

With support from the Chemical Measurement and Imaging Program, Prof. Mary Kraft and her group at the University of Illinois are developing a new approach to chemical imaging of organized structures like membranes using high-resolution secondary ion mass spectrometry. The work entails development and optimization of functionalized protein-specific affinity labels, metabolic labeling strategies, cell preservation protocols, and statistical methods to evaluate component co-localization. The method will significantly impact our understanding of how plasma membrane organization affects cellular function, and the molecular interactions that produce this organization. The project will provide interdisciplinary training of graduate students. Other educational activities focus on promoting the professional development of K-12 math and science teachers and educating middle and high school students about the chemical interactions that drive biological membrane organization. Results will be incorporated into laboratory activities and supplemental teaching materials for middle and high school students that will be provided to teachers through the Nano-CEMMS Center at the University of Illinois.