Mechanisms of cell-cell contact induced activity modulation in myeloid leukocytes (B02)

髓系白细胞细胞间接触诱导活性调节的机制（B02）

• 批准号: 200805233
• 金额: --
• 依托单位: Medizinische Klinik und Poliklinik I (Kardiologie) - Campus Großhadern
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200805233?language=en
• 关键词: Mechanisms; cell; contact; induced; activity

The activity of immune cells is regulated by external factors derived from pathogens or tissue injury, but also by direct cell-cell contacts. Here, we will investigate how direct interactions between neutrophils and macrophages influence their mutual functional state by performing in vivo calcium imaging. Next, we will determine the role of chemokines, integrins, and calcium channels in this interplay. Finally, we will manipulate calcium levels during cell-cell contacts to analyse the importance of calcium oscillations for this process. This will give new insights into inflammatory processes and how they can be targeted.

免疫细胞的活性受到来自病原体或组织损伤的外部因素的调节，但也受到细胞-细胞直接接触的调节。在这里，我们将研究如何直接之间的相互作用，中性粒细胞和巨噬细胞影响他们的相互功能状态进行体内钙成像。接下来，我们将确定趋化因子、整合素和钙通道在这种相互作用中的作用。最后，我们将在细胞-细胞接触过程中操纵钙水平，以分析钙振荡对这一过程的重要性。这将为炎症过程以及如何靶向它们提供新的见解。